Question

Two-step model of constitutive unregulated gene expression.

a) Write down the corresponding system of coupled ordinary differential equations (ODEs) based on mass action kinetics. Identify and explain which biological/biochemical process the “source” and “sink” terms in each equation describe.

b) State the parameters for steady-state mRNA and protein levels.

OM promoter (A) mRNA (M) protein (P) Figure 1. Two-step model of constitutive (unregulated) gene expression. Here the promoter (A) is a constant and represents a gene.

Answer 1

A. Source and sink :- the coupling of mass and quality flow using the properties of temperature mass concentration viscosity And density .

B. PARAMETERS :- Splicing, capping, and addition to poly A tail.  

Operons are activated in the presence of transcription factors. mRNA Are produced that are short lived.

In genetics promoter is a sequence of DNA To which Proteins binds initiate transcription of single mrna downstream to it.

Promoter is recognised by RNA POLYMERASE and some sigma factors. A binding site for RNA POLYMERASE 1. Transcibe gene encoding ribosomal RNAs.

1.Their is balance between mRNA translation and protein removal this is reflect by steady state protein level.

2. There is study of quantification method of the measurement of raw correlation that is between the arrangement of mRNA and proteins.

3. Using mRNA data sheet with more than 5000 gene there is light and dark gray bars .

Steady state of mRNA is determined by 2 process :- 1. Synthesis and 2. Degradation.  

For cytochrome c oxidase there is increase in activity . Low temperature reduces decay rate . Change in mRNA levels are arise from changes in synthesis.  

Nonsense mRNA decay and stability controls.

Post transciptionally controls.