You’re trying to PCR amplify a gene, but your gel shows that there are two bands...
You’re trying to PCR amplify a gene, but your gel shows that there are two bands instead of only one like you expected. You know for certain that there no homologous genes in the genome from which you’re amplifying, so you assume that the issue must be coming from your primers or PCR conditions. Briefly describe 2 different strategies for solving the problem and how they work.
Homework Answers
One strategy to solve the problem is that,the annealing temparature might be too low.At low temperatures the primers starts hybridising. So increasing the annealing temperature can solve the problem.
Other strategy is that,the DNA concentration might be too low. If more primers are used, the DNA concentration will be varying at diferent sites. This problem can be solved by using a better template concentration
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You’re trying to PCR amplify a gene, but your gel shows that
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A) what are the ley reagents needed to PCR amplify this specific region of the mouse...
A)
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solve for gel preparation and PCR mastermix calculations with
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II. Amplify the gene of interest using the PCR, verify the PCR products on the gel electrophoresis, analyze the gel. 4. By the next morning, the PCR amplification of all the samples is over, and you set up gel electrophoresis to check for the PCR products. Results: Lane 1 Alexandra - one band Lane 2 Olga - one band Lane 3 Tatiana - one band Lane 4 Maria - one band Lane 5 Anastasia - one band Lane 6 Alexey...
A)
what are the ley reagents needed to PCR amplify this specific
region of the mouse genome.
B) diagram the PCR fragment and the site of the RLFP on this
fragment woth lengeth in kb indicated.
C) what is the map distance between the deafness and his
RFLP?
diagram genotype and phenotype in crossing scheme snd explain
your answer.
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DNA-binding transcription factor
(Dbp1p) on transcribed genes. You fragment DNA from wild type
cells and immunoprecipitate
Dbp1p with an antibody to it. Then you PCR amplify the DNA
associated with Dbp1p using
different sets of primers for a particular gene (G3PD). One set
of primers was specific for the
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amplified the 3’ end of the open...
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diagnostic value in determining whether a patient has a mutation
within a specific DNA region. You know that this DNA segment of the
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steps for understanding
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