Question

A) what are the ley reagents needed to PCR amplify this specific region of the mouse genome.

B) diagram the PCR fragment and the site of the RLFP on this fragment woth lengeth in kb indicated.

C) what is the map distance between the deafness and his RFLP?

diagram genotype and phenotype in crossing scheme snd explain your answer.

In mice, PCR amplification of a specific DNA fragment revealed a simple autosomal RFLP. In some individuals, the fragment could be cut with BamH1 yielding a 2kb and 5kb bands when run a on gel. In other individuals, the fragment was not cut with BamH1 resulting in a 7kb band when run on a gel. A mouse homozygous for the 7kb RFLP and a recessive mutant causing deafness is crossed to a mouse homozygous for the 2kb,5kb RFLP and normal alleles at the deafness gene. The F1 individuals from this cross are mated with a mouse homozygous for the 7kb RFLP and a recessive mutant causing deafness. Of the progeny from this cross that are deaf, 60 produce a 7kb PCR product and 6 produce 7kb, 5kb and 2kb PCR products.