Question

To determine if your unknown carries out anaerobic respiration, which of the tests below would you perform? Nitrate broth Nutrient Gelatin Phenylalanine agar Catalase test

Answer 1

Ans : Nitrate broth

Description : Nitrate broth is used to determine the ability of an organism to reduce nitrate (NO3) to nitrite (NO2) using the enzyme nitrate reductase. It also tests the ability of organisms to perform nitrification on nitrate and nitrite to produce molecular nitrogen.

Anaerobic respiration is respiration using electron acceptors other than molecular oxygen (O2). Although oxygen is not the final electron acceptor, the process still uses a respiratory electron transport chain.

In aerobic organisms undergoing respiration, electrons are shuttled to an electron transport chain, and the final electron acceptor is Oxygen. Molecular oxygen is a highly oxidizing agent and, therefore, is an excellent electron acceptor. In anaerobes, other less-oxidizing substances such as Nitrate (NO3−), Fumarate, Sulphate (SO42−), or Sulphur (S) are used. These terminal electron acceptors have smaller reduction potentials than O2, meaning that less energy is released per oxidized molecule.

Note: Sir please give me positive rating .this rating boost up me .i do more effectively my work.

THanks