The important enzymes involved are recombination activating genes 1 and 2 (RAG), terminal deoxynucleotidyl transferase (TdT), and Artemis nuclease, a member of the ubiquitous non-homologous end joining (NHEJ) pathway for DNA repair.
Recombination Activation enzymes initiate rearrangement and recombination of genes of immunoglobulin and T cell receptor. There are two types of RAG found in lymphocytes , RAG1 and RAG2 which are essential for generation of mature B and T cell. They recognise RSS signal present on the gene and cleave the non coding region of DNA between the two exons to be joined. Their absence can lead to severe immunodeficiencies like SCID
Other enzymes are involved in the process are DNA-dependent protein kinase (DNA-PK), X-ray repair cross-complementing protein 4 (XRCC4), DNA ligase IV, non-homologous end-joining factor 1 (NHEJ1) for joining two sequences after recombination by RAG.
help wsecond typed question V(D)J recombination of germline encoded variable genes is required to produce the...
help wsecond typed question as opposed to b cells t cells recognize composite ligands on the surface of the bodys cells via the t cell receptor (TCR) name the molecules recognized by TCR and describe their basic structure Monocytes move from the systemic circulatory system into general connective tissues, where they differentiate into what phagocytic cell type?
help wsecond typed question thyroglobulin is a tissue specific antigen produced by follicular cells of the thyroid and used in thyoid gland. however thyroglobulin expression can be detected in some thymic epithelial cells due to activity of a unique transcription factor what is the transcription factor? also what would be the consequence of the absenxs of this transcription factor in the peripheral T cell repertoire Monocytes move from the systemic circulatory system into general connective tissues, where they differentiate into...