Question

immunology lab questions

In your own words whatsangen? These proteins of the immune response bind pathogens, and these Troond pathogans are subsequently recognized and destroyed by phagocytic cells: Some specific type of interactions between the protein and its and recessary for Any Chromatography to beco m e Out The interaction between a land and a proton must be broken to Interaction? the protein being purified What did we in our Affinity Chromatography to be heroe n d Question 14 In the Affinity Chromatography experiment after you analyzed the SOS-PAGEgal, what was the most abundant protein in the flow through fraction? Question 15 This immunoglobulin is responsible for allergic reactions and our body also producest during infection by parasites such as worms S PUGOT

Answer 1

Any foreign substance, it can be protein, a new organ or toxin which triggers a immune response in the body is called antigen.

Major histocompatibility complex (MHC) are proteins found on the surfaces of cells that help the immune system recognize foreign substances.

Affinity chromatography is a method of separation of biochemical mixture based on a highly specific interaction. For example antigen and antibody, enzyme and substrate, receptor and ligand,

For elution most common method is to lower the pH with 0.1 M glycine HCl, pH 2.5-3.0, which disrupts the ionic and hydrogen bonds between the antigen and antibody.

In flow through segment all other things or proteins will be present except the one which has been seperated by affinity chromatography.

Immunoglobulin E is responsible for allergic responses.