What is the absorption maxima for DNA? What does the ratio 260/280 signify? How does the...
Homework Answers
The Maximum absorption of DNA can be defined as the wavelength at which a sampleshows maximum absorbance. It is also known as Lamda Max, There are several important ion of a substance is needed. There are various factors which describes why maximum absorbance is needed
- As wavelength of every substance is distinct it helps in characterising the properties.
- Gives information about the analyte electronic structure.
- Gives accurate results and minimises deviations from Lambert Beer Law.
The ratio A260/A280 signifies the a variety of DNA/protein mixtures in the sample. As absorbance of DNA is calculated at wavelength of A 260 nm. As during the quantification of DNA other impurities in the form of protiens are also extracted and to check the impurities A280 is also considered as proteins shows maximum absorption at A280 nm. So for the sake of purity and depict best results the ratio A260/A280 is consideered by measuring the sample absorbance at these two wavelenghts.
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What is the absorption maxima for DNA? What does the ratio
260/280 signify?
How does the...
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What happens to the distance between maxima when the gratings per mm are increased? How does...
What happens to the distance between maxima when the gratings per mm are increased? How does changing the wavelength of the laser affect the distances between maxima?
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What happens to the distance between maxima when the gratings per mm are increased? How does changing the wavelength of the laser affect the distances between maxima?
es and that they 7) Suppose that one human rypote has the normal number of DNA proofreading and repair enzymes and that the function correctly, while another, for some reason, has no such functioning enzymes. As growth and developmen. proceeded, what differences in DNA structure in the cells and overall organism functioning would you expect to f... between the two? 8) In what normal human cells does telomerase function? In what disease does telomerase function in the cells (much to...
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How does the reserve ratio change if the Fed increases the required reserve ratio? How does the currency-deposit ratio change if people decide to keep more of their money in cash rather than depositing it? If people decide to hold zero currency (, meaning the currency- deposit ratio goes to zero), what is the relationship between the money supply and the monetary base (what is the money multiplier)? If people decide to hold all of their money as currency and...
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