Radioactive probes attach to the complimentary base during the process of gel electrophoresis. For instance a...
Radioactive probes attach to the complimentary base during the process of gel electrophoresis. For instance a C would attach to a G and a T would attach to an A (nucleic acids).
Homework Answers
The radioactive DNA probe is a single-stranded DNA molecule that is complementary to the sequence of interest. The DNA is separated on agarose gel, transferred to a solid nylon membrane made of nylon. The DNA is denatured to create single-stranded DNA, and the probe is incubated with the membrane. The probe is usually labelled with a 32Phosphorus isotope, incorporated in ATP. After washing to reduce non-specific binding, the membrane is put with x-ray film, and the binding of the probe is detected. Thus, the probe is tagged and released the single-stranded DNA. We can track it to see whether the probe binds to the array of DNA, and identify the sequence of interest.
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Radioactive probes attach to the complimentary base during the
process of gel electrophoresis. For instance a...
Question 5 The size of a piece of DNA can determine how fast or slow it travels during gel electrophoresis. True...
Question 5 The size of a piece of DNA can determine how fast or slow it travels during gel electrophoresis. True False Question 6 Radioactive probes attach to the complimentary base during some DNA lab processes. For instance a C would attach to a G and a T would attach to an A (nucleic acids). True False Question 7 How could/might all of this type of information at some point in time impact you personally (or your family members)? (Consider...
QUESTION 7 0.5 points Save Arrower In forensic DNA analysis a process that uses gel electrophoresis,...
QUESTION 7 0.5 points Save Arrower In forensic DNA analysis a process that uses gel electrophoresis, it separates DNA segments by Deoxyribonucleic acids. nitrogenous bases. O molecular weight. O short lines 0.5 points Save Anne QUESTION 8 The term that refers to the DNA replication occurring in opposite directions on each strand is O antiparallel Gene Isotope Conservative 0.5 points Save Answer QUESTION 10 The strands of DNA are arranged such that the bases protrude inwards such that a base...
If you stopped gel electrophoresis halfway during the running time, what would you expect? not all...
If you stopped gel electrophoresis halfway during the running time, what would you expect? not all the fragments have separated from each other in the gel yet the smallest fragments will still be in the loading well of the gel the largest fragments will be at the opposite end of the gel
During gel electrophoresis, DNA travels towards the positive charge. Including the substance agarose in the gel...
During gel electrophoresis, DNA travels towards the positive charge. Including the substance agarose in the gel allows for visualization of DNA under UV light. Below is an image of a gel run with a single DNA sample (ane 2) and a DNA ladder (lane 1). Using the DNA ladder reference included to the right, it can be concluded that two fragments labeled C&D in the DNA sample are approximately 6.0 Lane 1 2 kilobases and 3.0 kilobsases, respectively. Laai Auxilwa...
Biologists use gel electrophoresis to sort DNA segments by size. DNA segments are placed at one...
Biologists use gel electrophoresis to sort DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively charged (with a charge of two electrons per base pair). When you “run the gel” you are generating an electric field by connecting anodes and cathodes at the ends of the gel. This causes the negatively charged DNA segments to move towards the positive electrode. After running the gel, smaller DNA segments have moved farther from the...
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are...
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively chargod (with a charge of two electrons per base pair). When you "run the gel" you are generating an electric field by connecting anodes and cathodes at the ends of the gel This causes the negatively charged DNA segments to move towards the positive electrode. After nunning the gel, smaller DNA segments have moved...
DNA Electrophoresis: a) The DNA reaches terminal velocity quickly during electrophoresis and stays in that state....
DNA Electrophoresis:
a) The DNA reaches terminal velocity quickly during
electrophoresis and stays in that state. Solve for the terminal
velocity for the viscous and drag force case.
b) Which of these terminal velocities depend on the size of the
DNA molecule? How does the terminal velocity change with molecule
size? ( does terminal velocity go up or down as the size gets
bigger?)
c) Based on your calculations is the resistive force on a DNA
molecule during electrophoresis best...
The PCR procedure can be performed with fluorescently labeled primers and polyacrylamide gel electrophoresis (PAGE). What...
The PCR procedure can be performed with fluorescently labeled
primers and polyacrylamide gel electrophoresis (PAGE). What would
be the advantage of this procedure over agarose gel analysis?
PAGE allows for higher resolution than the agarose gel
procedure.
The PAGE procedure is faster than that using agarose gel
electrophoresis.
The labeled primers are less expensive because they are used at
a lower concentration in the reaction mix.
The PAGE procedure is less prone to contamination
Case Study 12-2 A 7-month-old child...
parts a,b, c please 3. Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis a. After pouring the...
parts a,b, c please
3. Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis a. After pouring the SDS polyacrylamide gel, you realize that instead of 2 ml, you used 4 ml of 30% acrylamide/bisacrylamide solution for the preparation of the separating gel (in both cases for 5 ml, total gel volume). How would this affect the separation of your proteins during SDS PAGE? Explain your answer! b. You have to remake the gel and are now making sure just to add...
The picture to the right below is of an actual acrylamide gel that has undergone electrophoresis...
The picture to the right below is of an actual acrylamide gel that has undergone electrophoresis for DNA fingerprinting. The molecular weight ladder (marker) is on the left, and the sizes of the different marker bands are indicated. a. How do the bands that are lettered A, B, C, D and E look to you? Would you describe them as pretty clear and defined, blurry and hard to distinguish, or a combination? KD I 1 b. Do you think that...
QUESTION 7 0.5 points Save Arrower In forensic DNA analysis a process that uses gel electrophoresis, it separates DNA segments by Deoxyribonucleic acids. nitrogenous bases. O molecular weight. O short lines 0.5 points Save Anne QUESTION 8 The term that refers to the DNA replication occurring in opposite directions on each strand is O antiparallel Gene Isotope Conservative 0.5 points Save Answer QUESTION 10 The strands of DNA are arranged such that the bases protrude inwards such that a base...
If you stopped gel electrophoresis halfway during the running time, what would you expect? not all the fragments have separated from each other in the gel yet the smallest fragments will still be in the loading well of the gel the largest fragments will be at the opposite end of the gel
During gel electrophoresis, DNA travels towards the positive charge. Including the substance agarose in the gel allows for visualization of DNA under UV light. Below is an image of a gel run with a single DNA sample (ane 2) and a DNA ladder (lane 1). Using the DNA ladder reference included to the right, it can be concluded that two fragments labeled C&D in the DNA sample are approximately 6.0 Lane 1 2 kilobases and 3.0 kilobsases, respectively. Laai Auxilwa...
Question 4-12 points Biologists use gel electrophoresis to sont DNA segments by size. DNA segments are placed at one end of a gel. DNA is negatively chargod (with a charge of two electrons per base pair). When you "run the gel" you are generating an electric field by connecting anodes and cathodes at the ends of the gel This causes the negatively charged DNA segments to move towards the positive electrode. After nunning the gel, smaller DNA segments have moved...
DNA Electrophoresis:
a) The DNA reaches terminal velocity quickly during
electrophoresis and stays in that state. Solve for the terminal
velocity for the viscous and drag force case.
b) Which of these terminal velocities depend on the size of the
DNA molecule? How does the terminal velocity change with molecule
size? ( does terminal velocity go up or down as the size gets
bigger?)
c) Based on your calculations is the resistive force on a DNA
molecule during electrophoresis best...
The PCR procedure can be performed with fluorescently labeled
primers and polyacrylamide gel electrophoresis (PAGE). What would
be the advantage of this procedure over agarose gel analysis?
PAGE allows for higher resolution than the agarose gel
procedure.
The PAGE procedure is faster than that using agarose gel
electrophoresis.
The labeled primers are less expensive because they are used at
a lower concentration in the reaction mix.
The PAGE procedure is less prone to contamination
Case Study 12-2 A 7-month-old child...
parts a,b, c please
3. Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis a. After pouring the SDS polyacrylamide gel, you realize that instead of 2 ml, you used 4 ml of 30% acrylamide/bisacrylamide solution for the preparation of the separating gel (in both cases for 5 ml, total gel volume). How would this affect the separation of your proteins during SDS PAGE? Explain your answer! b. You have to remake the gel and are now making sure just to add...
The picture to the right below is of an actual acrylamide gel that has undergone electrophoresis for DNA fingerprinting. The molecular weight ladder (marker) is on the left, and the sizes of the different marker bands are indicated. a. How do the bands that are lettered A, B, C, D and E look to you? Would you describe them as pretty clear and defined, blurry and hard to distinguish, or a combination? KD I 1 b. Do you think that...
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