Question

D See Hint In enolase, the reaction mechanism uses lysine at position 345 as a base to remove a proton from 2-phosphoglycerate as the first step. However, this results in an increased negative charge on the carboxylate group of the resulting carbanion intermediate. There are Mg2+ ions near the carboxylate part that function to - the stability of the carbanion intermediate prior to glutamic acid at position 211, donating a proton to the intermediate in order to form the final product, phosphoenolpyruvate.

Answer 1

In enolase, the reaction mechanism uses lysine at position 345 as a base to remove a proton from 2-phosphoglycerate as the first step. However, this results in an increased negative charge on the carboxylate group of the resulting carbanion intermediate. There are two magnesium lons near the carboxylate part that function to increase the stability of the carbanion intermediate prior to glutamic acid at position 211, donating a proton to the intermediate in order to form the final product, phosphoenolpyruvate.

MECHANISM FOR CONVERSION OF 2-PHOSPHOGLYCERATE TO PHOSPHOENOLPYRUVATE:

Enolase Enolase Ag. 2'Ag . H:0 -]ܢܘ'" 60260 05 o ܘܓܘ Och2 CC-CH2 Mg2+ 68 Enolase _H Ha Enolase 6-H _H-0 ab -ܪܽܝܽܪܽܦܽ of C - 0 f cr0 CH2 _ CH2 _ G ܙ CH2 CH2 Enolase 345-Lys Glu211-Enolase Enolase 345-Lys Glu211-Enolase