Question

How you would determine an enzyme regulation? Below is a series of results from tests of one particular enzyme. List all the potential methods of regulation still possible after each test. The options are: "genetic" control, zymogenic, phosphorylation, competitive inhibition, noncompetitive inhibition, positive allosteric regulation, and irreversible inhibition. 1. The enzyme is always present in the cell. 2. All tests only slow enzyme activity. 3. Adding more substrate does not increase reaction rate. 4. There seems to be a form of the enzyme that is slightly larger than another form.

Answer 1

In this case, experimental observation suggest the type of enzyme regulation is Zymogenic because in this case enzyme presents all the time in cell either active or inactive form. all test only slows the enzyme activity shows that enzyme are in the inactive form. adding more substrate enzyme activity does not increase because the enzyme reaches Vmax. and inactive and active form of the enzyme has a different molecular weight and size.