Question

1. Serum albumin          pI=4.8 (blue)

2. Cytochrome C:          pI =10.2                    

3. Hemoglobin (rabbit): pI=6.8

4. Myoglobin:                  pI=7.2

The pH of the Tris-Glycine buffer used in Part A is 8.6, what is the net charge on the protein molecules in the samples? Which electrode should each sample migrate toward during the electrophoretic separation? (4 points)