Homework Answers
| Protein | Direction traveled | Net charge |
| Cytochrome C | towards cathode | positive |
| Myoglobin | towards anode | negative |
| hemoglobin | towards anode | negative |
| serum albumin | towards anode |
negative |
At pH 6
| Protein | Direction traveled | Net charge |
| Cytochrome C | towards cathode | positive |
| Myoglobin | towards cathode | positive |
| hemoglobin | towards cathode | positivtive |
| serum albumin | towards anode |
negative |
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Protein Electrophoresis Isoelectric Point Proteins, Cytochrome C = 102 Myoglobin = 7.2 Hemoglobin = 6.8 Serum...
1. Serum albumin pI=4.8 (blue) 2. Cytochrome C: pI =10.2 &
1. Serum albumin pI=4.8 (blue) 2. Cytochrome C: pI =10.2 3. Hemoglobin (rabbit): pI=6.8 4. Myoglobin: pI=7.2 The pH of the Tris-Glycine buffer used in Part A is 8.6, what is the net charge on the protein molecules in the samples? Which electrode should each sample migrate toward during the electrophoretic separation? (4 points)
6. You are given a mixture of proteins that you analyze by standard 2-D electrophoresis, with...
6. You are given a mixture of proteins that you analyze by standard 2-D electrophoresis, with the following results for isoelectric points and apparent molecular weights: protein A (Mr 110,400; pl 4.60), protein B (Mr 10,100; pl 6.93), protein C (Mr 65,200; pI 7.84), and protein D (M 25,000; pI 8.15) a. After the 2-D separation, which protein will be in the upper left quadrant of the gel. given that e cathode-proximal end of the isoelectric focusing gel was on...
0.5. What fraction of the offsprings of two parents with Sickle cell trait would you expect...
0.5. What fraction of the offsprings of two parents with Sickle cell trait would you expect to have Sickle cell anemia? Q.6. List the major steps you undertake for performing ELISA? 0.7. Describe an ELISA procedure you'll undertake to determine if a person is infected with AIDS virus? Q.3. In the two protein gel electrophoresis labs done in this class, proteins were separated based on their net charge or their size (weight). Explain similarities and differences between these two laboratory...
er two Clini For resis is per unofixation protein electrophores on serum from a patient with...
er two Clini For resis is per unofixation protein electrophores on serum from a patient with the 7,formed on of mulipl would most likely revecal which of the feing uestions myeloma. The r Which of the following acute-phase reactant proteins decrases during inflammation a. Transferrin mon type of multipl y reveal whi a. An IgG monocional band b. Oligoclonal bands C. Significant p-γ bridging d. An lgM monoclonal band b. a-Antitrypsin d. Fibrinogen 2. Which of the following refers to...
6. You are given a mixture of proteins that you analyze by standard 2-D electrophoresis, with the following results for isoelectric points and apparent molecular weights: protein A (Mr 110,400; pl 4.60), protein B (Mr 10,100; pl 6.93), protein C (Mr 65,200; pI 7.84), and protein D (M 25,000; pI 8.15) a. After the 2-D separation, which protein will be in the upper left quadrant of the gel. given that e cathode-proximal end of the isoelectric focusing gel was on...
0.5. What fraction of the offsprings of two parents with Sickle cell trait would you expect to have Sickle cell anemia? Q.6. List the major steps you undertake for performing ELISA? 0.7. Describe an ELISA procedure you'll undertake to determine if a person is infected with AIDS virus? Q.3. In the two protein gel electrophoresis labs done in this class, proteins were separated based on their net charge or their size (weight). Explain similarities and differences between these two laboratory...
er two Clini For resis is per unofixation protein electrophores on serum from a patient with the 7,formed on of mulipl would most likely revecal which of the feing uestions myeloma. The r Which of the following acute-phase reactant proteins decrases during inflammation a. Transferrin mon type of multipl y reveal whi a. An IgG monocional band b. Oligoclonal bands C. Significant p-γ bridging d. An lgM monoclonal band b. a-Antitrypsin d. Fibrinogen 2. Which of the following refers to...
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