Question

46-49 pleas help asap

ou employ CRISPR to target a DNA double strand break to your gene of interest. The break is then repaired via SA (Synthesis dependent strand annealing) using the sister chromatid as a template for repair. What change in DNA sequence would occur at your target gene? a. A deletion b. No change at all c. A change converting it to whatever allele is present on the homolog d. A chromosome arm exchange (crossover) with the homolog e. An insertion of your CRISPR gene at the target gene 47) The arginine biosynthetic operon would be expected to be regulated as follows: a. Lots of arginine -> repressor-arginine biosynthetic genes b. Lots of arginine -> activator -> arginine biosynthetic genes c. Lots of arginine - repressor - arginine biosynthetic genes d. Lots of arginine - activator -> arginine biosynthetic genes e. Either a ord would work. 48) A signal transduction pathway is illustrated in figure SIGNAL below, where the signal is boredom. It outlines the series of regulatory processes that affect a student's decision to go to the movies and/or eat at a Thai restaurant. What is the student's response to SIGNAL? Go to the movies, but no Thai food Having Thai food, but no movie Thai food and a movie Neither Thai food nor movie d. a sense of a. 49) In the signal transduction pathway illustrated below, the student is defective in factor "B" responsibility). As a result, the student will: ALWAYS go to the movies, regardless of "signal". Only go to the movies in the absence of signal. Only go to the movies in the presence of signal d. NEVER go to the movies. e. Only go to the movies if Thai food is present. ZA 4 B Go to the movies Signal D - Get Thai food

Answer 1

46) C) A change converting into whatever allele is present on the homolog

It is a non-cross over technique. After the repair, there will be gene conversion. In this repair, DNA is synthesized with the help of the homologous chromosome. So the genes in the homolog will be copied to the new DNA piece.

47. Answer is A

In the presence of Arginine, the repressor is activated, ArgR. This will inhibit transcription. Arginine synthesis is negatively regulated.

48. B) Having Thai food, but no movie.

The signal activates A. A inhibits B. So the plan pf going to movies is inhibited.

The signal activates C. C inhibits D. This activates the plan of eating Thai food.

49.A) Always go to the movie, regardless of the signal.

Here B is which controls the action of going to the movie. It is inactive, so it won't prevent the action. So the student will go to the movie, even if there is a signal or not. (In the absence of signal, B will not be activated in normal case).