Question

no explanation needed only answer for multiple choice

4. Choose the best pair of primers to use in amplifying the target DNA molecule shown below. Primers Target DNA molecule a. 5-TGATAC-3', 3-GCTGAC-5' 5'-GTCAGCATCGGAACTGATAC-3 b. 5-GTCAGC-3', 5' CATAGT 3' 3'-CAGTCGTAGCCTTGACTATG-5 c. 5'-TGATAC-3', 3-ACTATG-5' d. 3-ACTATG-5', 5-GTCAGC-3' e. 5'-CGACTG-3', 5-ACTATG-3' 5. A mutation in murt gene would result in which of the following? a. An increase in the frequency of GC to TA transversion mutations b. An increase in the frequency of AT to GC transition mutations c. An increase in the frequency of TA to GC transition mutations d. An increase in the frequency of AT to GC transversion mutations e. An increase in the frequency of insertion and deletion mutations 6. Which of the following results would you expect to encounter if you carry out a blue white cloning assay in media that contains both glucose and lactose? a. Only blue colonies b. Mostly white colonies with some blue colonies c. Mostly blue colonies with some white colonies d. Only white colonies e. An equal mixture of white and blue colonies

Answer 1

Question 4. Answer is option A because primer used is complement to template strand and hence nucleotide in template strand are complementary to the primer sequence given in option a hence a is correct answer.

Question 5. Answer is D that is an increase in the frequency of AT to GC transversion mutation. Because the biological role of mutT protein is to prevent the A.G mispairs during replication.

Question no.6 answer is C that is mostly blue colonies and some white colonies are present because there is disruption of lacZ gene which result in decrease in white colonies amd increase in blue colonies.