Question

YOUCHSULUI About Courses Help Home My Courses de This course Question 11 Complete 0.00 points out of 1.00 Prof. Weimb's lab in MCDB on UCSB's campus studies polycistic kidney disease. One of his researchers recently published their research that focused on how the renal tubule deals with blockages to the filtrate/future urine flow. See the summary below Flag question Healthy tubule Normal flow • Crystal occludes tubule • Disrupted flow 1. Activation of mTOR, STAT3 • Tubule dilation by hypertrophy Dilated tubule • Re-established flow • Crystal passed to urine Crystal formation in these rat kidneys was by giving the rats hydroxyproline or glyoxylate (chemical precursors that fosters formation of calcium oxalate crystals in the renal tubule). Below are fluorescent micrographs (from Indirect fluorescence of the renal tubule). What can you conclude by analyzing these results? Control + OH-Pro + Glyoxylate TL DAPI GOS

Answer 1

The answer to the given question has been solved as under;

The correct option is : option c) Rats with calcium oxalate crystals enlarge the diameter of both their proximal convoluted tubule and collecting duct renal tubules, as it is evident from the fluorescent micrographs. Both CALB1 and LTL protein detected by the antibodies showing red and green fluorescence respectively show an increase in diameter.

Other options are wrong because;

A) both glyoxalate and hydroxyproline treated rata show increased diameter of collecting tubules.

B) same secondary antibody would have given same colour fluorescence

D) primary antibodies detect epitopes on proteins ans not mRNA.

E) Only C is correct.