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Question 12 Complete 0.00 points out of 1.00 P Flag question In addition to microscopy, the Weimbs lab then analyzed whether crystal deposition, and the resulting tubule dilation, required protein kinase cascade activity. In essence, the tubules detect obstruction, activate phosphorylation cascades (comprised of protein kinases - remember, these are enzymes that often phosphorylate themselves and phosphorylate other proteins) that carry the external stimulus to the cell's nucleus and changes gene expression, resulting in tubule dilation To prove this, the Weimbs lab did western blots with antibodies that detect phosphorylated kinases (as shown as p-PROTEIN NAME). The two protein kinases they looked at are S6 and STAT3, well-known protein kinases. What do the blots below demonstrate? NaOx - = no sodium oxalate given to rats NaOx + = rats fed sodium oxalate (and resulting crystal deposition in kidney tubules) Note: you do not need to memorize these results! I just want you to be able to interpret western blots. 3 h + 1 d + NaOx: - - + + + + p-S6 Total S6 P-STAT3 Total STAT3

Answer 1

The right answer here is option c. Phosphorylated S6 and STAT3 show higher intensity bands at 3h, compared to those in the lanes without NaOX treatment.

Option a is wrong as total protein of S6 and STAT3 show more or less uniform intensity signals irrespective of NaOX treatment.

Option b is wrong as Actin bands are of uniform intensity in all samples.

Option d is wrong too as the phosphorylated S6 band intensity is even higher than that of the 3h treatment.