If primer-dimers were to form as a product of the TAS2R38 PCR, what size would you...

Question

Question

If primer-dimers were to form as a product of the TAS2R38 PCR, what size would you predict them to be? d. O

PCR Master Mix GoTaq DNA Polymerase 2.5 μM forward primer: 5-AACTGGCAGAATAAAGATCTC 2.5 ㎂Λ reverse primer: 5-AACACAAACCATCACS

Add a comment Improve this question Transcribed image text

Answer 1

Answer #1

For the given primer sequences the primer-dimer product size for TAS2R38 can be achieved approximately at 353 to 400 bp with given low salt concentration.

Add a comment

Answer 2

Similar Homework Help Questions

2. You are performing PCR under the following conditions: 1. Each PCR reaction contains 50 pl...

2. You are performing PCR under the following conditions: 1. Each PCR reaction contains 50 pl final volume II. Each PCR reaction contains: 1x PCR buffer, 1.5 mM MgCl2, 0.2 mM dNTP mix, 0.2 MM sense primer, 0.2 uM anti-sense primer, 1.5 units of Taq polymerase and 2 ul of DNA template. [all concentrations are final] III. Stock solutions of the following reagents are available: 10x PCR buffer, 25 mm MgCl2, 10 mM dNTP mix, 10 M of sense primer,...
CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in th...

CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in this experiment you must add each ingredient separately (unlike the "master-mix" we used in our own experiment). In this experiment, each PCR reaction tube contained the following ingredients added with a micropipetor... 10 HL of 5x Buffer 6 μ1 of 25 mM MgCl2 solution μL of 10 mM dNTP mix 1.5 μL oligonucleotide primer mix 0.5 HL Taq DNA Polymerase stock solution 28...
Prepare a PCR reaction using the following recipe. 5x buffer..............................5 μl 25 μM forward primer..........2 μl,...

Prepare a PCR reaction using the following recipe. 5x buffer..............................5 μl 25 μM forward primer..........2 μl, 25 μM reverse primer..........2 μl 5 ng/μl Template..................1 μl 2.5 mM (each) dNTPs..........4 μl Water....................................30 μl Phusion polymerase.............1 μl Q6. What are the final concentrations of the buffer, the forward primer, the template, and the dNTPs in the PCR reaction? Leave all concentrations in the same type of units they were provided in (x, molarity, or weight per volume). Remember the equation C1 x...

4 total reactions 10. Complete this Master Mix table for 3 DNA samples, a positive control,...

4 total reactions 10. Complete this Master Mix table for 3 DNA samples, a positive control, negative control, and an extra reaction for pipetting error. Show your work. 4 pts) Master C Mix Conc. Of Final Stock solution L/Rxn Number of Total L (total per Reactions needed tube) for master mix PCR buffer Water dNTP mix MgCl2 F Primer 20X 1X | | 25 mM2.5 mM 10uM | 0.1 μΜ 100 ml 100 μΜ RPrimer | 10uM | 0.1μΜ Taq...
solve for gel preparation and PCR mastermix calculations with steps for understanding TBE Buffer Calculations Determine...

solve for gel preparation and PCR mastermix calculations with steps for understanding TBE Buffer Calculations Determine the mass of the following reagents for a 10X stock 700mM of Tris Base (157g/mol) 887mM of Boric Acid (62g/mol) 25.7mM of EDTA (292g/mol) Dissolve in 750ml of DIH.O and bring to volume (IL) Calculate the dilution of your 10x stock for a 1X working stock. Remember you only need IL of working stock for a single experiment. Gel Preparation Calculations You need to...

If primer-dimers were to form as a product of the TAS2R38 PCR, what size would you...

Homework Answers

Add Answer to:
If primer-dimers were to form as a product of the TAS2R38 PCR, what size would you...

Post as a guest

Earn Coins

2. You are performing PCR under the following conditions: 1. Each PCR reaction contains 50 pl...

CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in th...

Prepare a PCR reaction using the following recipe. 5x buffer..............................5 μl 25 μM forward primer..........2 μl,...

4 total reactions 10. Complete this Master Mix table for 3 DNA samples, a positive control,...

solve for gel preparation and PCR mastermix calculations with steps for understanding TBE Buffer Calculations Determine...

If primer-dimers were to form as a product of the TAS2R38 PCR, what size would you...

Homework Answers

Add Answer to: If primer-dimers were to form as a product of the TAS2R38 PCR, what size would you...

Post as a guest

Earn Coins

2. You are performing PCR under the following conditions: 1. Each PCR reaction contains 50 pl...

CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in th...

Prepare a PCR reaction using the following recipe. 5x buffer..............................5 μl 25 μM forward primer..........2 μl,...

4 total reactions 10. Complete this Master Mix table for 3 DNA samples, a positive control,...

solve for gel preparation and PCR mastermix calculations with steps for understanding TBE Buffer Calculations Determine...

Add Answer to:
If primer-dimers were to form as a product of the TAS2R38 PCR, what size would you...