11. If pB253 were cleaved with BamHI and Sacl, what are the sizes of the two...

Question 1

Question

11. If pB253 were cleaved with BamHI and Sacl, what are the sizes of the two DNA fragments that would result? (show units)

668 Xhoi- 689 Hindill - 701 Eco RI 707 Pst. 719 Bam HL - pB253.xdna + 3334 bp 1101 Spel 1107 Xbal. 1131 Sacl.

science biology

Add a comment Improve this question Transcribed image text

Answer

Answer #1

+719 (Bam H1) 3334 2922 1412 (1131-71 1131 (dacz, ((13-719=414) (3334- 414-2922) 2922 & fragments , 0 0 . . 412

Please rate high.

Add a comment

Answer

Similar Homework Help Questions

If the target DNA (the 3.266 Kb E. coli genomic Bam HI fragment) has the same restriction sites on each end, there are t...

If the target DNA (the 3.266 Kb E. coli genomic Bam HI fragment) has the same restriction sites on each end, there are two possible orientations for the target DNA to insert into the plasmid. The following restriction enzymes would cut the 3.266 kb Bam HI genomic fragment containing the RecA gene once or twice or not at all. In the tables below, list the expected DNA fragment sizes for the two possible orientations. Round the DNA fragment sizes to...

Question 2

If the target DNA (the 3.266 Kb E. coli genomic Bam HI fragment) has the same restriction sites on each end, there are t...

If the target DNA (the 3.266 Kb E. coli genomic Bam HI fragment) has the same restriction sites on each end, there are two possible orientations for the target DNA to insert into the plasmid. The following restriction enzymes would cut the 3.266 kb Bam HI genomic fragment containing the RecA gene once or twice or not at all. In the tables below, list the expected DNA fragment sizes for the two possible orientations. Round the DNA fragment sizes to...

11. If pB253 were cleaved with BamHI and Sacl, what are the sizes of the two...

Homework Answers

Add Answer to:
11. If pB253 were cleaved with BamHI and Sacl, what are the sizes of the two...

Post as a guest

Earn Coins

If the target DNA (the 3.266 Kb E. coli genomic Bam HI fragment) has the same restriction sites on each end, there are t...

11. If pB253 were cleaved with BamHI and Sacl, what are the sizes of the two...

Homework Answers

Add Answer to: 11. If pB253 were cleaved with BamHI and Sacl, what are the sizes of the two...

Post as a guest

Earn Coins

If the target DNA (the 3.266 Kb E. coli genomic Bam HI fragment) has the same restriction sites on each end, there are t...

Add Answer to:
11. If pB253 were cleaved with BamHI and Sacl, what are the sizes of the two...