A mutant E. coli strain does not express lacZ, even in the presence of lactose. What...
A mutant E. coli strain does not express lacZ, even in the presence of lactose. What is a possible genotype of the cells?
Group of answer choices
lacI+ lacP+ lacO+ lacZ+ lacY+ lacA+
lacl+ lacP+ lacO+ lacZ+ lacY+ lacA+
lacI- lacP+ lacO- lacZ+ lacY+ lacA-
lacI+ lacP+ lacO+ lacZ- lacY- lacA+
lacI- lacP+ lacOc lacZ+ lacY+ lacA+
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A mutant E. coli strain does not express lacZ, even in the
presence of lactose. What...
my teacher was useless with the method for doing these problems can someone please guide me...
my teacher was useless with the method for doing these
problems can someone please guide me in the right direction. dont
do all of them, but if you do at least explain how you came to that
conclusion. thank you
u
6. For E. coli strains with the lac genotypes show below, use a plus sign (+) to indicate the synthesis of B-galactosidase and permease and a minus sign (-) to indicate no synthesis of the proteins. Lactose absent Lactose...
20862a a A mutant E. coli strain is found that does not synthesize any enzymes in...
20862a a A mutant E. coli strain is found that does not synthesize any enzymes in the presence or absence of lactose for allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. ones where proteins bind DNA genes and regulatory sequences och Operator o Lacy o Lacz o Laca promoter MacBook Air * जीप
help please as soon as possible A mutant E. coli strain is found that does not...
help please as soon as possible
A mutant E. coli strain is found that does not synthesize any enzymes in the presence or absence of lactose (or allolactose). W mutation(s) can lead to this outcome? The lac operon is shown here as a guide. fones where proteins bind DNA genes and regulatory sequences Operator La repressor B-galactosidase B-galactos de transactylase proteins promoter Operator Lacy Lac A laci o Lacz
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the...
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the wild-type gene or site that is fully functional, and – designates a deletion of that gene or site; lacO+ means all operators (O1, O2 and O3) are functional; assume all other genes and sites not listed are wild-type and functional (including lacA). In some cases, a plasmid containing a wild-type functional gene or site was transformed into the E. coli strain. The strains were...
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the...
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the wild-type gene or site that is fully functional, and - designates a deletion of that gene or site; lacot means all operators (01, O2 and 03) are functional; assume all other genes and sites not listed are wild-type and functional (including lacA). In some cases, a plasmid containing a wild-type functional gene or site was transformed into the E. coli strain. The strains were...
A partial diploid E. coli strain does not express lacZ either in the presence or in...
A partial diploid E. coli strain does not express lacZ either in the presence or in the absence of lactose. Which of the following genotypes are consistent with this phenotype? A. ISO+Z+/F'I-OCZ+ B. I-O+Z-/F'I-OCZ+ C. ISO+Z+/F'I-OCZ- D. I+O+Z+/F'I-OCZ+ E. ISO+Z-/F'I-OCZ+
1, (12 pts.) You are fortunate to obtain a strain of E. coli from the Pasteur...
1, (12 pts.) You are fortunate to obtain a strain of E. coli from the Pasteur Institute labeled #348, preserved from the work of Jacob & Monod. The genotype of the strain is Lac LacP Laco' Lacz' LacY. The Lacf mutation results in resistance to allolactose allosteric inhibition so it is always bound to the LacO operator. The LacY is a mutation resulting in zero permease activity. Lucky for you, today's echnology allows you to test this bacterial strain to...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences I lacl promoter operator lac Z l ac Y La repressor B-galactosidase B-galactoside transacetylase proteins Operator Laci promoter Lac Y Lac A Lacz
25. You take your lac/ strain and transform in two F' plasmids to conduct a complementation...
25. You take your lac/ strain and transform in two F' plasmids to conduct a complementation test. The first F' plasmid contains the entire lac operon from a strain that is lacz, while the second F' plasmid contains the entire lac operon from a strain that is lacY. You test the ability of the transformed bacteria to grow on media where the sole carbon source is either glucose or lactose, and obtain the following results: Lac t mutant compl lacZ...
4. Monod's group figured out how the different parts of lac operon work by constructing "meroploidl...
4. Monod's group figured out how the different parts of lac operon work by constructing "meroploidl E. coli cells. These have two copies of each of the lac operon genes, one in their chromosome and one on a plasmid* (actually a separate chromosome fragment introduced via conjugation), so they are "partial diploids." Many different versions of these were constructed, with different combinations of mutations in the chromosomal and/or plasmid- encoded genes. +/- indicate active/inactive gene products. O® = "constitutively active...
my teacher was useless with the method for doing these
problems can someone please guide me in the right direction. dont
do all of them, but if you do at least explain how you came to that
conclusion. thank you
u
6. For E. coli strains with the lac genotypes show below, use a plus sign (+) to indicate the synthesis of B-galactosidase and permease and a minus sign (-) to indicate no synthesis of the proteins. Lactose absent Lactose...
20862a a A mutant E. coli strain is found that does not synthesize any enzymes in the presence or absence of lactose for allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. ones where proteins bind DNA genes and regulatory sequences och Operator o Lacy o Lacz o Laca promoter MacBook Air * जीप
help please as soon as possible
A mutant E. coli strain is found that does not synthesize any enzymes in the presence or absence of lactose (or allolactose). W mutation(s) can lead to this outcome? The lac operon is shown here as a guide. fones where proteins bind DNA genes and regulatory sequences Operator La repressor B-galactosidase B-galactos de transactylase proteins promoter Operator Lacy Lac A laci o Lacz
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the wild-type gene or site that is fully functional, and - designates a deletion of that gene or site; lacot means all operators (01, O2 and 03) are functional; assume all other genes and sites not listed are wild-type and functional (including lacA). In some cases, a plasmid containing a wild-type functional gene or site was transformed into the E. coli strain. The strains were...
1, (12 pts.) You are fortunate to obtain a strain of E. coli from the Pasteur Institute labeled #348, preserved from the work of Jacob & Monod. The genotype of the strain is Lac LacP Laco' Lacz' LacY. The Lacf mutation results in resistance to allolactose allosteric inhibition so it is always bound to the LacO operator. The LacY is a mutation resulting in zero permease activity. Lucky for you, today's echnology allows you to test this bacterial strain to...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences I lacl promoter operator lac Z l ac Y La repressor B-galactosidase B-galactoside transacetylase proteins Operator Laci promoter Lac Y Lac A Lacz
25. You take your lac/ strain and transform in two F' plasmids to conduct a complementation test. The first F' plasmid contains the entire lac operon from a strain that is lacz, while the second F' plasmid contains the entire lac operon from a strain that is lacY. You test the ability of the transformed bacteria to grow on media where the sole carbon source is either glucose or lactose, and obtain the following results: Lac t mutant compl lacZ...
4. Monod's group figured out how the different parts of lac operon work by constructing "meroploidl E. coli cells. These have two copies of each of the lac operon genes, one in their chromosome and one on a plasmid* (actually a separate chromosome fragment introduced via conjugation), so they are "partial diploids." Many different versions of these were constructed, with different combinations of mutations in the chromosomal and/or plasmid- encoded genes. +/- indicate active/inactive gene products. O® = "constitutively active...
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