Draw and label the major parts of the following MS instruments:
- Time-of-flight mass spectrometer (TOF-MS)
- Three-dimensional quadrupole ion trap MS
Draw and label the major parts of the following MS instruments: - Time-of-flight mass spectrometer (TOF-MS)...
Time of Flight (TOF), Triple Quadrupole (QQQ), ion trap, Fourier-transform ion cyclotron resonance (FT-ICR) ion trap. Rank in order of decreasing accuracy
An acceleration voltage of 30.0 kV is used to accelerate ions into the 1.54 m flight tube of a time‑of‑flight (TOF) mass spectrometer. The frequency at which spectra can be recorded is dependent on how long it takes the slowest ion to travel from the source to the detector. If you wish to scan up to ?/?m/z 6801 at what frequency (spectra/s) could spectra be recorded if a new spectrum begins each time the slowest ion reaches the detector? frequency:................spectra/s
For each of the following mass analyzers, associate these systems with their appropriate operating pressure, detector type, experiment type, and nominal resolution. (For example, a QMS is often paired with a CEM detector and not a MCP). Note, that a single characteristic may be assigned to more than one mass analyzer. Quadrupole Mass Spectrometer ______________________________________________________________ Time of Flight Mass Spectrometer ______________________________________________________________ Triple Quadrupole Mass Spectrometer ______________________________________________________________ Orbitrap ______________________________________________________________ Ion Trap Mass Spectrometer ______________________________________________________________ Channel Electron Multiplier Image Charge Detection Microchannel...
Suppose that you have a time-of-flight mass spectrometer with a detector that can clearly resolve ion signs that are at least 14.0 nanoseconds apart in arrival time. Given a flight tube length of 1.40 m total field-free distance from source to detector, and a source with an acceleration voltage that extends over a 2.5 cm distance, a) determine the source voltage required to ensure that two proteins with masses 2237.5 amu and 2239.8 amu are adequately resolved, i.e. have a...
Electrospray time‑of‑flight (TOF) mass spectrometry was used to analyze the eluate from a high‑performance liquid chromatography (HPLC) separation. The mass spectrum of one chromatographic peak, containing a protein of unknown molecular mass, displays MHnn+ peaks at m/z= 6636.908, 6194.524, 5807.480, 5465.932, 5162.360, and 4890.726. Find the average molecular mass (M) of the neutral protein and its standard deviation.
Electrospray time‑of‑flight (TOF) mass spectrometry was used to analyze the eluate from a high‑performance liquid chromatography (HPLC) separation. The mass spectrum of one chromatographic peak, containing a protein of unknown molecular mass, displays MHn+n peaks at m/z= 6710.365, 6263.085, 5871.757, 5526.428, 5219.496, and 4944.856. Find the average molecular mass (M) of the neutral protein and its standard deviation.
In mass spectrometry, a sample’s molecules are ionized in a vacuum and then exposed to a uniform electric field created by a high-voltage plate in the acceleration chamber. The electric field accelerates the ions until they arrive at the next section of the device, designated as the separation chamber. In this section, the drifting ions are sorted by their mass-to-charge ratio (m/q). The separation chamber in a time-of-flight mass spectrometer (TOF-MS) is linear and has no electric or magnetic fields....
Mass Spectrometry Part A: Match each statement with the term it most closely matches. A Hard ionization B Electrospray Ionization (ESI) C Molecular ion peak D Ion-Trap E Fast atom bombardment (FAB) F Time of Flight (TOF) G Base peak H Quadrupole I Soft ionization J Matrix-Assisted Laser Desorption/Ionization (MALDI) K Magnetic sector 1. ___ This ionization technique is used to analyze large, non-volatile molecules. The analyte is dissolved in a liquid matrix and placed on a target where it...
3. Draw the major and minor products for each of the following reactions. Label major or minor if more than one product. (14 pts) Br DBU 1. TsCl, pyridine CH3CH2OH 2. methoxide ion HO 1. BH, 2.1,0. OH d. HC CHỊCH5OH
Draw the major and minor products for each of the following reactions. Label major or minor if more than one product. CH2 + HBr 1. CH3CH20 2. H2O