Question

.PH Time pH 3 pH 6 pH 7 pH 10 15 0.07 0.324 0.228 0.217 30 0.102 0.429 0.352 0.314 45 0.134 0.522 0.477 0.418 60 0.165 0.607 0.596 0.517 75 0.195 0.683 0.705 0.609 90 0.223 0.737 0.807 0.695 105 0.249 0.827 0.897 0.775 120 0.275 0.889 0.983 0.85

The numerical data above has been graphed. Based on this data, please answer the following questions:

1.What was your predication for the optimum pH of the glucose oxidase?
Is your result what you predicted? If not give an explanation as to why.

2.At which pH did glucose oxidase have the highest enzyme activity?
In your own words, explain why glucose oxidase activity was highest at this pH

3. Which pH was the most acidic one in this experiment? What effect did this pH have on glucose oxidase enzyme activity (basically, how does the glucose oxidase activity at this pH compare to the activity at the other pHs)? In your own words, explain why this pH had the effect that you observed.

4. Which pH was your most basic one? What effect did this pH have on glucose oxidase enzyme activity (basically, how does the glucose oxidase activity at this pH compare to the activity at the other pHs)? In your own words, explain why this pH had the effect that you observed.

Answer 1

1) From the values, slope was calculated.

pH	Slope
3	0.0025
6	0.0085
7	0.0089
10	0.0077

When slope versus pH was ploted-

0.01 0.009 0.008 0.007 0.006 0.005 0.004 0.003 0.002 0.001 0 0 2 4 6 8 10 12

According to the curve, the optimal pH is pH=7. The peak is at pH= 7, that is, the value is reached at pH=7. Thus the optimal pH of glucose oxidase is 7 according to the plot.

The known value for the optimal pH of glucose oxidase is 5.5. It has a broad pH range of 4 to 7.

2) According to the plot, the pH at which glucose oxidase shows highest activity is ph= 7. This is because most of the physiological pH is 7.

3) The acidic pH in this experiment was pH=3. The activity of glucose oxidase at pH=3 is the lowest.

4) The most basic pH in this experiment is 10. The activity drops at this pH.