Question

A protein with an isoelectric point of 9.1 in a buffer of pH 5.2 will A)...

A protein with an isoelectric point of 9.1 in a buffer of pH 5.2 will

A) Have a net negative charge

B) Be neutral

C) Have a net positive charge

Please explain, thank you.

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Answer #1

C) Have a net positive charge

At its isoelectric point peptide would have no charge whereas below its isoelectric point peptide would have a positive charge and above its isoelectric point, the peptide would have a negative charge. The pH of the buffer is 5.2 which is lower than the isoelectric point of the peptide so the peptide would have a net positive charge.

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