The isoelectric point of Trypsin is 10.5. Is the net charge of Trypsin at pH 7.4 positive?
I believe the correct answer to be:
YES
The isoelectric point is the pH at which the charge on the molecule is 0 or neutral. The lower pH result in positive overall charge while the higher pH result in overall negative charge.
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The isoelectric point of Trypsin is 10.5. Is the net charge of Trypsin at pH 7.4...
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The isoelectric point (pI) of a peptide is the pH at which the peptide does not migrate in an electric field. Since the peptide is zwitterionic, there are the same number of positive charges as negative charges on the peptide population. The pI can be estimated fairly accurately (within 0.1 or 0.2 pH units) from the pK values of all the proton dissociable groups in the peptide. Using pK values from the table at the right, estimate the pI value...
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