Hi can you check/help me with these? And please explain if you can! Thank you!
These questions are about Ion-exchange Chromatography
1. The charge on the column is negative
a. Would the protein KLGVRQYWRRRRPLYWTV bind to it?
2. Does the buffer action work by a difference in ionic strength?
3. 
4. T/F: You will likely get more fractions of DNP-glycine than the other two proteins.
True because DNP-glycine goes through very fast because it is small.
5. T/F: Cytochrome c is the only protein that binds to the column.
True because Cytochrome c is the positively charged protein.
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