Question

8 dno6-u SIH l2 NetID: Name: 13. Which one of the forms of amino acids labeled a-e would you NOT expect to find in aqueous solution? d. на о CH2 H2 CH H2 NH c=NH2 NH2 он tutamune 19. In a mixture of the five soluble proteins listed below, which should flow out of a gel filtration column second? a. Serum albumin, relative molecular mass (M) 68,500 b. Cytochrome c, M 13,000 c. Immunoglobulin G, M 145,000 d. Ribonuclease A, M 13,700 e. RNA polymerase, M, 450,000

can you explain these two to me please :)

the answer for 13 is B but i don't understand why. for 19 the answer is C but i again i dont get why. ..

Answer 1

13) In option b only the COOH and NH₂ are not ionize (means they are not form COO^- and NH₄⁺) . Generally amino acids in aqueous state they form COO^- and NH₄⁺

^{19) in gel filtration protien which is haiving high molecular weight that elute or come out first . So RNA polymerase come out first and then immunoglobulin come out as second.}

^{in your question they ask second protein come out so option b correct}