Question

If you want to reproduce the shuttling experiment as performed during week 3. you will need to purchase Cycloheximide (CHX) from one of three potential suppliers. CHX has a molar mass of 281.35 g/mol and can be dissolved in DMSO, water, or EtOH but with different solubilities. You will need to use CHX at a final working concentration of 17 μg/mL in a 150 mm dish with a total media volume of 14 mL.

Determine how much of the stock solution purchased from each company you would need (in μL) to add to the media in the 150 mm dish to obtain the final working concentration of CHX.

Give your answers in μL using the scientific notation.

a. Company A supplies CHX dissolved in DMSO at 100 mg/ mL:

Volume needed in μL

b. Company A supplies CHX dissolved in water in 2% (w/v) solution

Volume needed in μL:

c. Company A supplies CHX dissolved in EtOH at 50 mM

Volume needed in μL:

Answer 1

company » providing conath I = 100 ng/mL = = 10suglme EHX concth at final working = 17 luglme tz volume of solh needed = 14m2 va Total art - 170g/m2 x lume - 23ong E xv, 2x ve 10 ug/m2 xv,= 17 ug/m2 x 14 m2 Vi= 230 m2 105 230x155 m2 - 2. 38 ML y company 7 92 coneth = 2% who = ag in 100 mL = Rog12 E = 2.0x10-5 ug/2 > V,= 17 ugime x lum2 2x100 ug/m2 2.0x 10 = 0.0l19 ug/m2 m2 = lloguz

coneth = 50 mm = 50 xlo-3 M = 50X10-3 x 281.35 912 = 14067.5 ug/m2 Tixvi = axua VI 17 ug Ime x lume 14067.5 ugline V = 16.91 M2