Describe the reaction mechanism for the enzyme aldolase, include the nucleophiles and the electrophiles in the reaction. What would happen to the reaction mechanism if the active site Lysine were mutated to alanine?
Describe the reaction mechanism for the enzyme aldolase, include the nucleophiles and the electrophiles in the...
Question 6 2 pts Fructose 1,6-P2 aldolase functions in both glycolysis and gluconeogenesis. Starting with DHAP in Schiff base linkage with the active site lysine of aldolase, show how that intermediate reacts with G 3-P to form fructose 1,6-P2 in Schiff base linkage at the active site. Use B: and HA as necessary. Make sure that you indicate mechanism with curly arrows, and draw each step out, but don't go all the way to the end of the reaction where...
PLEASE WRITE OUT A MECHANISM SHOWING ARROWS (NUCLEOPHILES AND ELECTROPHILES) FOR THIS REACTION. I HAVE INCLUDED THE REACTANTS, REAGENTS AND THE PRODUCT AS WELL IN THIS PICTURE. > Mechanisms OH TG HIGO (produt) Br mgbr -H bryetgo Dry Etao
please help thanks VI You are studying the mechanism of catalysis for an enzyme and find that mutating an active site lysine to glutamate has no effect on initial binding, but it significantly decreases the rate at which the enzyme catalyzes its reaction. Predict how this mutation (Lys Glu) is affecting catalytic rate in three sentences or fewer. (Hint, what might the lysine be doing to facilitate catalysis?) LMS stabilizes transition State
PT1. Which of the following does not describe a mechanism that cells use to regulate enzyme activity? Explain. (5 points) Cells control enzyme activity by the binding of small molecules Cells control enzyme activity by phosphorylation and dephosphorylation Cells control the diffusion rates of substrate to enzymes Cells control the rate of enzyme degradation Cells control the rate of enzyme synthesis Cells control the targeting of enzymes to specific organelles PT2. Which of the following properties of an enzyme is...
Under typical conditions, many essential biochemical reactions proceed so slowly that life could not exist without the presence of enzymes. Enzymes increase reaction rates through a wide variety of mechanisms. These mechanisms generally utilize the following strategies: improving the nucleophiles and electrophiles present in the catalytic R groups or substrates; stabilizing the extra electron density of the leaving group; and stabilizing transition states. Proteases are enzymes that break down proteins by hydrolyzing peptide bonds. Chymotrypsin is a protease found in...
The molecule 1, is well known mechanism based inhibitor for one of the PLP containing enzyme. The molecule can adopt either pathway A or B, and can lead to I or II adduct formation. One adduct was formed from 1 covalently linked to pyridoxal 5-phosphate (PLP) (I) while the other adduct was formed with the inhibitor covalently linked to Lysine246, the active site lysine. NH 0 o,po HN н. Lys HN Co.
The second part of the mechanism for histidyl-tRNA-synthetase is shown below. Active site residues are in black and the substrates are shown in green. Examine the image and complete the following questions. RNAH A76 Arg113 Gin 127 Glu131 M-o Histidy-Adenylate Arg259 Tyr264 c 1. Describe the role of Arg259 in the active site of histidyl- RNA-synthetase. Proton anor acid 2. If the substrates for this enzyme are histidine, ATP, and tRNAHs. Describe overall (not mechanism steps) what must have happened...
B. Describe the following reaction mechanism, circle any amino acid residues in the diagram that are acting as acids, bases, electrophiles, or nucleophiles. S, s Ss NH, NH, TS, R ~NH-R TS2 R NH-R R-C-NH-R His H 1 E E E: ES I, 12 S, S: NHS GH, NES 29 TS, TS, R- + H2O -R'NH2 R-C H His H SH B B E E E, E4 Et Ει E4 Ig 14
identify all reaction mechanism components and provide a forward synethesis and retrosynethesis for the product created. label all nucleophiles and electrophiles. show and explain how the product becomes stabilized. 7. Please provide a reasonable mechanism for the transformation below. Be sure to include all intermediates, formal charges, and arrows depicting electron flow. Then, explain the source of stability for the nucleophile in the reaction. (8 points) Org. Lett. 2000 2, 2717. Me. Me. Me Me Me Me Me
1) What role does the active site lysine play in the mechanism of CO2 fixation in Rubisco? Hypothesize what effect the mutation of this lysine to arginine would have on the protein.