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6. Experiments comparing the expression and silencing mechanisms of cloned eukaryotic gene "A" via the lac...
-Which elements are found in a eukaryotic promoter vs. a prokaryotic promoter? -what is the concept of (restriction enzyme produced) DNA fragment separation by gel electrophoresis -what are the steps and process of thermal cycling? -what is the restriction enzyme(s) and how do you know when they leave blunt or sticky ends (ie. XbaI, SmaI, EcoRI, BamHI)? -The lac and trp operons (form a figure showing the operon). - What is RNA silencing involvee (in general, what is RNA interference;...
A genetically engineered plasmid Ficarries all of the elements of the lac operon, but replaces the structural genes with the reporter gene green fluorescent protein (GFP). The repressor allele cannot bind lactose, and the OC allele cannot be bound by the repressor. Given the following genotypes and growth media that does not contain glucose, indicate in the table below whether you expect to see b-galactosidase activity and glowing green colonies of E. coli by selecting Yes or No. growth media...