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uestion 5 hich of the following represents the correct sequence of events in bacterial translational initiation? O30S binds Shine-dalgarnoN-formylMet-tRNA binds AUG5QS binds 2nd aminoacyl-tRNA binds in the P site OSOS binds Shine-dalgarno .. N-formylMet-tRNA binds AUG 30S binds 2nd aminoacyl-tRNA Sinds in the A site O30S binds Shine-dalgarno .> N-formylMet-tRNA binds AUG 50S binds 2nd aminoacyl-tRNA binds in the A site 30S binds Shine-dalgarno>50S binds N-formylMet-tRNA binds AUG>2nd aminoacyl-tRNA binds in the A site
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Ans.) 30S binds shine Dalgarno -> 50S binds N-formylMet-tRNA binds AUG -> 2nd aminoacyl-tRNA binds in the A site. This is the correct sequence of events in bacterial translational initiation.

-First of all the 30S subunit binds to the shine Dalgarno sequence present on mRNA, then the N-formylMet-tRNA binds to the initiation codon AUG with complementary base pairing which is followed by binding of 50S large subunit to N-formylMet-tRNA. Then the second upcoming amino-acyl-tRNA binds in the A-site to proceed elongation of the polypeptide chain.

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