Homework Answers
No individual 1 is not their sibling as the restriction digestion pattern are different from the other two because of a phenomenon known as restrcition fragment length polymorphism (RFLP) analysis.
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Below is a Southern blot probe for gene ABC for three individuals in lanes 1, 2...
draw a southern blot in which genomic DNa frow WW rabbits, Ww rabbits pink, and ww...
draw a southern blot in which genomic DNa frow WW rabbits, Ww
rabbits pink, and ww rabbits white has been digested with EcoRI and
hybridized to probe A
3. You have discovered a coat color gene ("W" gene) in rabbits that has two alleles, W and w. Rabbits that are homozygous for W (WW) are red. white. Heterozygous rabbits (Ww) are pink. Rabbits that are homozygous for w (ww) are You have also identified a DNA sequence of about 100...
19. Two children are investigated for the expression of a gene (D) that encodes an important...
19. Two children are investigated for the expression of a gene
(D) that encodes an important enzyme for muscle
development. The results of the studies of the gene and its product
follow.For child 2, the enzyme activity of each stage was very low
and could be estimated only at approximately 0.1 unit at ages 1, 2,
3, and 4
a. For both children, draw graphs representing the developmental
expression of the gene. (Fully label both axes.)
b.How can you explain...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers to locations where the restriction endonuclease EcoRI cuts the DNA, and Pst refers to locations where the restriction enzyme Pst cuts the DNA. Potential restriction sites are numbered 1-6. Distances between restriction sites are shown on the bottom scale in base pairs (bp). The thick line represents the part of the molecule that has homology with a probe. Eco Pst Eco Pst Eco Pst...
8. The accompanying photo shows a Southern blot of the FMR-1 gene (Xq27.3) for eight patients...
8. The accompanying photo shows a Southern blot of the FMR-1 gene (Xq27.3) for eight patients (lanes 1-8). Lane 9 is a normal female control. Which of the following is the most likely explanation for the pattern observed in lane 6? 1 2 3 4 5 6 7 8 9 10 Inactivated X a This is a female with fragile X syndrome. b. This is a male with fragile X syndrome. c. This is a male with Huntington disease. d....
The map below illustrates three alleles in a genome segment. The alleles differ in the number...
The map below illustrates three alleles in a genome segment. The alleles differ in the number and location of restriction sequences. Restriction digestion and Southern blotting with the molecular probe whose hybridization location is indicated results in detection of a single DNA band for each allele. a. List the size of the DNA bands that would be detected for each of the following genotypes: D^2 D^2: D^2 D^3: D^3D^3: b. Restriction-digested DNA from two organisms is analyzed by Southern blotting....
Biotech Workshop HW 2 1. Compare and contrast Southern blot and Western blot on basic technique...
Biotech Workshop HW 2 1. Compare and contrast Southern blot and Western blot on basic technique such as procedure, reagents, its usage and applications. (5points) 2. Discuss common features of Random Probe Labeling share with RT-PCR. What are major differences between these two techniques? (5points) 3. It is claimed by a pharmaceutical company that protein A' is beneficial to treat breast cancer since it promotes apoptosis. If you were put into charge of testing this, how would you design experiment?...
1. Which of the following RNAs can induce gene silencing? tRNA snRNA dsRNA rRNA miRNA 2....
1. Which of the following RNAs can induce gene silencing? tRNA snRNA dsRNA rRNA miRNA 2. Which vehicles are often used for gene therapy to carry a healthy gene? Bacteria Viruses Plastic capsules Both A and B Both A and C 3. A common method in biochemistry to detect and analyze proteins is: Northern blot Southern blot Western blot Eastern blot All of the above 4. The technique used to identify a specific DNA sequence in bacterial colonies is In...
QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated...
QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated in the gene map below. The 5' UTR and 3' UTR segments are each 25 bp long. Exons 1 thru 4 are 100, 200, 300, 400 bp long, respectively. Each intron is 200 bp each. The locations of the relevant EcoRI sites within the ACEX locus are indicated, but the location of other restriction enzyme sites (like BamHI) are not shown." EcoRI probe EcoRI...
ANSWER ALL QUESTIONS Questions 7-8: The figures below are a pedigree analysis of a genetic disorder...
ANSWER ALL QUESTIONS
Questions 7-8: The figures below are a pedigree analysis of a genetic disorder called small foot, and the corresponding molecular analysis of the small foot gene - associated Mstl-RFLP markers of Mary's family. Mary is individual 3. Parents Siblings NORMAL Each individual's small foot gene DNA was amplified, and digested with Msti. 1000 bp 400 600 600 bp 1 23 45 nividual PCR, Mst1 Digest DISEASE 400 bp A. The molecular summary of the small foot gene...
Not siblings Question #1 Based on the information below, calculate the probability that a child from...
Not siblings
Question #1 Based on the information below, calculate the probability that a child from Joseph and Josephine will have disease K Joseph and Josephine each have a brother and a sister with disease K. Joseph, Josephine and their parents do NOT have the disease. A. 4/9 B. 4/36 C. 2/3 D. 2/36
draw a southern blot in which genomic DNa frow WW rabbits, Ww
rabbits pink, and ww rabbits white has been digested with EcoRI and
hybridized to probe A
3. You have discovered a coat color gene ("W" gene) in rabbits that has two alleles, W and w. Rabbits that are homozygous for W (WW) are red. white. Heterozygous rabbits (Ww) are pink. Rabbits that are homozygous for w (ww) are You have also identified a DNA sequence of about 100...
19. Two children are investigated for the expression of a gene
(D) that encodes an important enzyme for muscle
development. The results of the studies of the gene and its product
follow.For child 2, the enzyme activity of each stage was very low
and could be estimated only at approximately 0.1 unit at ages 1, 2,
3, and 4
a. For both children, draw graphs representing the developmental
expression of the gene. (Fully label both axes.)
b.How can you explain...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers to locations where the restriction endonuclease EcoRI cuts the DNA, and Pst refers to locations where the restriction enzyme Pst cuts the DNA. Potential restriction sites are numbered 1-6. Distances between restriction sites are shown on the bottom scale in base pairs (bp). The thick line represents the part of the molecule that has homology with a probe. Eco Pst Eco Pst Eco Pst...
8. The accompanying photo shows a Southern blot of the FMR-1 gene (Xq27.3) for eight patients (lanes 1-8). Lane 9 is a normal female control. Which of the following is the most likely explanation for the pattern observed in lane 6? 1 2 3 4 5 6 7 8 9 10 Inactivated X a This is a female with fragile X syndrome. b. This is a male with fragile X syndrome. c. This is a male with Huntington disease. d....
The map below illustrates three alleles in a genome segment. The alleles differ in the number and location of restriction sequences. Restriction digestion and Southern blotting with the molecular probe whose hybridization location is indicated results in detection of a single DNA band for each allele. a. List the size of the DNA bands that would be detected for each of the following genotypes: D^2 D^2: D^2 D^3: D^3D^3: b. Restriction-digested DNA from two organisms is analyzed by Southern blotting....
Biotech Workshop HW 2 1. Compare and contrast Southern blot and Western blot on basic technique such as procedure, reagents, its usage and applications. (5points) 2. Discuss common features of Random Probe Labeling share with RT-PCR. What are major differences between these two techniques? (5points) 3. It is claimed by a pharmaceutical company that protein A' is beneficial to treat breast cancer since it promotes apoptosis. If you were put into charge of testing this, how would you design experiment?...
QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated in the gene map below. The 5' UTR and 3' UTR segments are each 25 bp long. Exons 1 thru 4 are 100, 200, 300, 400 bp long, respectively. Each intron is 200 bp each. The locations of the relevant EcoRI sites within the ACEX locus are indicated, but the location of other restriction enzyme sites (like BamHI) are not shown." EcoRI probe EcoRI...
ANSWER ALL QUESTIONS
Questions 7-8: The figures below are a pedigree analysis of a genetic disorder called small foot, and the corresponding molecular analysis of the small foot gene - associated Mstl-RFLP markers of Mary's family. Mary is individual 3. Parents Siblings NORMAL Each individual's small foot gene DNA was amplified, and digested with Msti. 1000 bp 400 600 600 bp 1 23 45 nividual PCR, Mst1 Digest DISEASE 400 bp A. The molecular summary of the small foot gene...
Not siblings
Question #1 Based on the information below, calculate the probability that a child from Joseph and Josephine will have disease K Joseph and Josephine each have a brother and a sister with disease K. Joseph, Josephine and their parents do NOT have the disease. A. 4/9 B. 4/36 C. 2/3 D. 2/36
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