The initial concentration of a protein sample is 25 μg/μl. Calculate the volumes of the stock...
The initial concentration of a protein sample is 25 μg/μl. Calculate the volumes of the stock protein and water (diluent) necessary to prepare a 1:5 dilution in a final volume of 4 mL. What is the diluted concentration?
Volume of Protein:
Volume of Water:
Resulting Concentration:
Homework Answers
I beleive the correct answer to be as follows:
1/5 = X/4,
X = 4/5 = 0.8mL.
So, you will need:
Volume of Protein: 800uL or 0.8mL
Volume of Water:3200uL or 3.2mL
Resulting Concentration: 5ug/ul.
feel free to leave a comment down below for any further query. good rating would be appreciated if you find my answer helpful. thank you.
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How do you find the initial concentration if you are only given the initial volume and final volume? I have tried using...
How do you find the initial concentration if you are only
given the initial volume and final volume? I have tried using the
V(i) x C(i) =V(f) x C(f) equation but it needs at least 3 figures
so I'm a bit lost right now.
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How do you find the initial concentration if you are only
given the initial volume and final volume? I have tried using the
V(i) x C(i) =V(f) x C(f) equation but it needs at least 3 figures
so I'm a bit lost right now.
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