Question

d. The images in panel d below show normal anaphase (first row) and anaphase in cells where dynein is not localized to the cell membrane. The chromosomes are fluorescently labeled. The kymograph in panel e shows the same data as in panel d (separation of sister chromatids durin anaphase). As you can see, if dynein is mislocalized, the chromosomes do not separate as mu the wild type control. Based on your knowledge of how dynein acts normally in anaphase B ex why you see aberrant chromosome movements in cells where dynein is mislocalized. Rubric (4): of dynein in normal Anaphase B (2). Explain why lack of dynein would cause the experimental result below (2). O Anaphase onsetEnd of late Anaphase ? End of early Anaphase O Cytokinesis control Type l ControlDynein mislocalized Dynein Dyneinmislocalized Typo!

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Aberrent chromosomes movement is seen when dyenin is mis localized because in this condition protein dyenin was not able to provide outward force which is responsible for separating the chromosomes at anaphase B stage.  

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