Question

3 2 1SDS-page run under non-reducing conditions:

Which lane(s) could be a heterodimer with no cystines?Why?

Which lane(s) could be a homodimer with no Cystines?Why?

Which lane(s) could be a multimeric protein made of 4 individual polypeptides? (No Cystine)

Which lane(s) could be a multimeric protein made of 3 differently sized polypeptides, 2 of which are connected by multiple cystines?

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Answer #1

Which lane(s) could be a heterodimer with no cystines?Why?

Ans: Lane: 1. 2 bands represent two polypeptides of heterodimer.

Which lane(s) could be a homodimer with no Cystines?Why?

Ans: Lane: 3. 1 band represents two polypeptides of homodimer.

                                                                          

Which lane(s) could be a multimeric protein made of 4 individual polypeptides? (No Cystine)

Ans: Lane: 2. 4 bands represent four polypeptides of multimeric protein.

Which lane(s) could be a multimeric protein made of 3 differently sized polypeptides, 2 of which are connected by multiple cystines?

Ans: Lane: 1. 2 bands represent two different polypeptides. One band is for a single peptide and another one is for two joined peptides by cystine.

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