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Why is there no simple method to detect the formation of gluose or the disappearance of...

Why is there no simple method to detect the formation of gluose or the disappearance of cellobiose?

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Answer #1

Cellobiose is made up of 2 glucose moieties. Both cellobiose and glucose have similar oxidative power, and use of dinitrosalicylic acid used for determining reducing sugars cannot differentiate between the two.

The 2 glucose moieties are linked in the 1-4 direction in cellobiose, hence cleavage of cellobiose, will lead to one molecule of glucose with a reducing end. Hence, DNS will not react with cellobiose.

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