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1.) what does it mean when lactose broths inoculated with 0.1ml of water show positive results?...

1.) what does it mean when lactose broths inoculated with 0.1ml of water show positive results?

2.) Is antibacterial soap better to use than regular soap when trying to prevent food poisoning? Are hand sanitizers better than antibacterial or regular soap?

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Answer #1

1) Lactose broths is inoculated with water inorder to determine the presence of coliform bacteria.The presence of a very few count of coliform bacteria indicates that the water probably contains no disease causing organism.Whereas a high number of coliforms indicate a very high probability for the water to contain disease causing organisms.

The water to be tested is first inoculated with lactose broth.If coliform bacteria present in it,they utilize the lactose present in the medium to produce acid and gas.The presence of acid is indicated by the color change of medium & the presence of gas is detected by the gas bubbles that are collected within the inverted Durham tube present in the medium.The total number of coliforms is determined by taking the count of tubes that produce positive result.Posituve result means there will be both color change and gas production.

2) Antibacterial soaps are no more effective than regular soap ,because it won't kill the healthy bacteria.

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