Question

The PCR process involves (multiple answer)

		the use of pressure to control the reaction
		the use of temperature to control the reaction
		multiple cycles to grow the copies
		the addition of fluorescing reagents
		the use of a high temperature stable enzyme
		the use of denaturized DNA

Answer 1

PCR involves:

• The use of temperature to control the reaction. In all the three stages of PCR, the temperature is changed either due to enzyme stability or annealing etc.
• Multiple cycles to grow the copies. Since in PCR a small segment of DNA is taken for amplification so to make a great number of copies of multiple cycles are needed. Also repeated cycle of heating and cooling is done to achieve the result of the process.
• The use of a high-temperature stable enzyme. Since for denaturation, the temperature is raised very high in which normal enzyme will denature or degrade so high-temperature stable enzyme is used.
• The use of denatured DNA. for PCR cycle to start amplifying DNA is firstly denatured with the help of high temperature.