The direct role of the DNA denaturation step in a PCR reaction is to Group of...
The direct role of the DNA denaturation step in a PCR reaction is to
Group of answer choices
provide the optimal temperature for the DNA polymerase used
separate the two DNA strands of the template
allow the primers to anneal to the DNA
2. Which of the following would be useful/necessary for an expression vector BUT not for a cloning vector?
Group of answer choices
origin of replication
regulatory sequences
antibiotic resistance gene
high copy number
Homework Answers
I believe the correct answer to be:
1) Option B) seperate the two DNA straas of the template.
As denaturation which occurs at high temperatures seperate the 2 DNA strands that were previously held together by hydrogen bonds.
2) Option B) Regulatory sequences.
As they play a role in transiption which is not needed by cloning vector but are needed by expression vector as they need to express the gene present in them.
feel free to leave a comment down below for any further query. good rating would be appreciated if you find my answer helpful. thank you.
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The direct role of the DNA denaturation step in a PCR reaction
is to
Group of...
This PCR step is called annealing. The annealing step follows the denaturation step: it is usually...
This PCR step is called annealing. The annealing step follows the denaturation step: it is usually the lowest temperature in the PCR. The temperature of this step varies with each PCR reaction because each primer has its own sequence and may not be an identical match to the DNA template strand. GC or CG have three hydrogen bonds and AT or TA have two hydrogen bonds. Higher annealing temperatures are more stringent and require a better match between primer and...
A protein binds a DNA sequence downstream of a promoter. This results in an increased rate...
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Now. you should be able to answer the following questions: • How the amplification will be...
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Which is not one of the elements needed to amplify DNA by polymerase chain reaction (PCR)?...
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1.When cloning a PCR product into a plasmid using restriction enzymes, the restriction enzyme recognition sequences...
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This PCR step is called annealing. The annealing step follows the denaturation step: it is usually the lowest temperature in the PCR. The temperature of this step varies with each PCR reaction because each primer has its own sequence and may not be an identical match to the DNA template strand. GC or CG have three hydrogen bonds and AT or TA have two hydrogen bonds. Higher annealing temperatures are more stringent and require a better match between primer and...
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
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