Question

8. PCR is used to. A Diagnose genetic disease 8 Solve cnmes C Sudy gene unction D. All of th C ONA as a template to form RINA D All of the above 7. PCR technique does not need A. Tag polymerase B Restriion encymes C Olgoucletide prmers C. A fragment of skin D. All of the above 9 PCR can be used in A Cloning B.Sequening C.Medical dagnosis&foric mine 0.PCR can make mullple copies ot A. DNA B RNA C Protein D Both A&B 11. Sequentially, what are the tree sleps of PCR? A Anneal Priners, Extend DNA. Denature ONIA B Denature ONA Extend Anneal Primers C.Denature DNA Anneal Primers, Extend DNA D.Extend ONA, Aneal Primers Denture 2 Small piece of DNA can be ampilified by A PCR B denaluration C hybridication D Polymenzation 3 Nucleotide triphosphates (dNTPs) are added to the growing DNA strand duing this phase 14 During teannealig step A Primer, bnd to he tealy separa dONAs B The 2ONAstra sepan, an emperatures C The ONA & master mis ane added together D. The new ONA product is nun on an agarose ge 15 Tag DNA polymerase does not? A Function at extremely high Temp B Add nudleoide trighosphates to form new DNA strand C Require a buffer enwironment to work D. Act as an exonuclease to out single-standN 16 When DNA copies are multiplied at a rapid rate, this is refeed to as amplification A Exponential B. Quick C. Moderate D Tmed 17 DNA molecules can be cut at speofic sequences using A UV light B.restriction nuclesses C DNA a A To prevent the nun olf of NIA of the ge 18.Why tracking dye was used in DNIA electrophoresis? C To make DNA denserD Boh&c B. To give positive charge to DNA A Methylene blue B. Agarose gelCTE buler C Alows the ONA Fragments to migrate through the agarose gel D All of the above through the mattr С Gel become brite & breaks DLageONAfrarent, get sto nthe manx EC&D 1 19 You used caution in using the tollowing chemical because it is a mulagen and carcingm D Ethidium bromide at7 B Conduct eletrioty 20 What purpose does the buffer serve in gel eectrophoress? A. Matain 21. What happens when too much agarose is added o the gel? A el wont soidly BONun o fas

Answer 1

6. D. All of the above

7. B. Restriction enzyme

8. D. All of the above

9. D. All of the above

10. A. DNA

11. C. Denature, Anneal, Extend

12. A. PCR

13. B. Annealing

14. A. primer binds to the newly separated DNAs

15. D. Act as a endonuclease to cut single stranded DNA

16. A. Exponential

17. B. Restriction endonucleases

18. D. Both A and C

19. D. Ethidium bromide

20. D. All of the above

21. D. larger DNA fragments get stuck in the matrix