1. c. Activator binding site
The protein sequence binds the activator binding site, which lies downstream of a promoter.
2. b. PCR can provide the information on abundance of specific gene targets.
The above statement is incorrect regarding PCR.
A protein binds a DNA sequence downstream of a promoter. This results in an increased rate...
If a protein binds to a DNA sequence downstream of a promoter, transcription is enhanced. Which site is likely to be attached?
Suppose there is a protein "A" which binds to a DNA sequence and increases transcription of the gene CBC23. In the presence of molecule "C", protein "A" changes structure and can no longer bind to the DNA sequence, resulting in reduced transcription of CBC23. What terms describe "A" and "C" respectively? Enhancer and activator Repressor and Effector Effector and activator Activator and enhancer Polymerase and activator Activator and effector
8. In transcription, A) mRNA is synthesized from DNA B) the starting point of synthesis is the promoter site C) RNA polymerase binds to the promoter during initiation D) the ending point of synthesis is the terminator site E) All of the above The tryptophan (trp) operon is actively transcribed (i.e. gene expression occurs) A) when there is excess tryptophan in the cell. B) when there is a lack of tryptophan in the cell. C) constitutively because the trp operon...
25. Promoter 26. Replication 27. RNA Polymerase F. Sequence of DNA that controls gene express G. binds an operator and stops gene expression in LAC operon by preventing RNA polymerase from binding gene and transcribing. H. Duplication of DNA in S phase of Interphase 28. Codon 29. Transgenic 30. Recombinant DNA 31. PCR 32. 33. 34. 35. 36. 37. 38. Plasmid Gene Therapy Gel electrophoresis DNA Profile DNA ligase GMO concern GMO benefit A. Analyzing STR patterns of blood or...
You wish to determine if there is a mutation somewhere within the promoter of the adult b-globin gene that could possibly be responsible for a case of b-thalassemia. Which ONE method, when compared to the same process performed on wild type DNA, would NOT provide you with information that could be consistent with this idea? A) Prepare a pair of 18 nucleotide long primers that hybridize upstream and downstream of the promoter, perform PCR, and sequence the resulting fragment B)...
17) Once a protein binds to DNA at a specific site a) Transcription may be blocked b) RNA polymerase may bind more efficiently c) RNA polymerase may be unable to transcribe the gene d) Transcription may be activated e) All of these choices may result
In rho-dependent transcription termination: the formation of a hairpin in the transcribed mRNA causes RNA polymerase to pause, facilitating termination. rho binds the mRNA, and when it makes contact with RNA polymerase, it assists with the removal of the mRNA from the DNA template. the rho factor binds to the -10 consensus sequence located in the promoter region to terminate transcription. a site within the poly(A) tail is cleaved which signals termination. the 3' untranslated region (3" UTR) is synthesized....
1. The map of the SheDoesntEvenGoHere operon is... P LAst Ics The promoter (P) region is the start site of transcription through the binding of the RNA polymerase molecule before actual mRNA production. The operon functions similarly to the lac operon, but has to do with the metabolism of muffins; therefore muffins are the effector molecules here. Just downstream of the promoter is the operator sequence (L). Lexus, coded by the Ast gene, is similar to permease while Secrets, coded...
You are using a ChIP assay to study the positioning of a DNA-binding transcription factor (Dbp1p) on transcribed genes. You fragment DNA from wild type cells and immunoprecipitate Dbp1p with an antibody to it. Then you PCR amplify the DNA associated with Dbp1p using different sets of primers for a particular gene (G3PD). One set of primers was specific for the TATA box region of this gene, (lane 1) another pair of primers amplified the 3’ end of the open...
please i need help with a, b, c this is the sequence 5’ATGTATTATTATTTTTTTGTTTTTTTTGCAATATATGCTAATGGATTGCTAAGAAATA AAGATCCTAACATTTTTGCGAG TAGCAATGATGAGATCATAGAAAATGATAAAAGTATGAATACCTTTGTTATGTCAAC AAATGGAAGTTTATATTTAAATA GTGATTTTAATTTAAATGAAGCATCCAACGAAAGCTTCTTAGAAAATTGCAATATCA ATAGTTGTGTAGATATAGGTCAT GAAAATGGCAACAAAATAAATAGTCAAGAAAATGAGCATGCTAAAAATAATAACA ACAGTAATAATAACAATTTAAAACC AGAATACAATAATAATAATAATAATTTAAAACCAGAATACAATAATAATAATTTAA AACCAGAGTACAATAATAACAATT-3’ 1. Polymerase chain reaction 5'- ATGTATTATTATTTTTTTGTTTTTTTTGCAATATATGCTAATGGATTGCTAAGAAATA AAGATCCTAACATTTTTGCGAG TAGCAATGATGAGATCATAGAAAATGATAAAAGTATGAATACCTTTGTTATGTCAAC AAATGGAAGTTTATATTTAAATA GTGATTTTAATTTAAATGAAGCATCCAACGAAAGCTTCTTAGAAAATTGCAATATCA ATAGTTGTGTAGATATAGGTCAT GAAAATGGCAACAAAATAAATAGTCAAGAAAATGAGCATGCTAAAAATAATAACA ACAGTAATAATAACAATTTAAAACC AGAATACAATAATAATAATAATAATTTAAAACCAGAATACAATAATAATAATTTAA AACCAGAGTACAATAATAACAATT-3' a) One strand of a chromosomal DNA sequence is shown above. How would you amplify and isolate a DNA fragment defined by the sequence shown in red, using polymerase chain reaction. Design PCR primers (Forward and Reverse primers, each 20 nucleotides long, that...