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Case Study B A policeman comes into the laboratory with a subpoena for the laboratory results. The Patient is accused of driving while intoxicated and caused a a car accident. A week later a defense attorney calls and requests information about the specimen results 1. The final report requires a confirmatory test, which is the method accepted in toxicology as the confirmation for alcohol analysis If the chromatogram (figure 3; shown below) corresponds to the calibrators used in the analysis of this patients sample and the final report indicated that ethanol was present in the sample which would be the retention time for the peak of the patient. 2.

terne Figure 3. Gas chromatogram of a mixture of acetaldehyde (1), methanol (2), acetone 3), ethanol (4), isopropanol (5), n-propanol (6), isobutanol (, and n-butanol (8) Column temperature: 60 °C, injection volume: 1.0 μ1 ; concentration of all volatiles: 0.8 gL; recorder output: 60 mv. In the figure 2 provided below indicate how high would be the peak of the patients sample by drawing a peak corresponding to a concentration of 3.5g/L; this level was the patients result in the confirmatory ethanol levels test. 3.

Figure 2. (a-e) Gas chromatograms of five calibrator solutions of ethanol in whole blood (1,2 3,4, and 5 g/L, respectively). Colunan temperature: 120 °C; injection volume: 2.0 μ L; injection depth: at least 1 5 cm beneath the surface of the glass beads in, the liner. Gas chromatogram of the same solution as under (e), but at an injection depth of 1 cm beneath the surface of the glass beads. Recorder output: 0.2V 4. Another patient showed in the chromatograph a peak identified as ethylene glycol. What metabolite of ethylene glycol is the major contributor to the characteristic high anion gap seen in this intoxication 5 List two other simple chemical lab tests (not osmolality) that would be pertinent to perform when patients have alcohol intoxications.

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If a person is accused for intoxicated driving, then the preliminary test is done by the breathe analyzers. Confirmatory tests in laboratory can be done by either taking the blood sample or the urine sample from the accused person.

In the blood test for confirming the intoxication, blood sample is drawn from the person at most by 12 hours in the vial coated with an anti-coagulant. the method involved for blood alcohol testing is carried out by Gas chromatography. Blood sample is injected in the gas chromatography column. Blood samples passes through the column and separated out alcohol present in the blood sample.The instrument or recorder then notes the amount of alcohol present in the form of electrical signals. After obtaining the electrical signals, the instrument then prepares a frequency chart according the the amount of the alcohol present.

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