Question

Please can someone help me out with the predictions.

Experiment: This “lab" will examine how disruption (mutation) of parts of a signaling pathway affect the results of activating that pathway. Specifically you will examine the effect of a factor on cell division in normal a cells ( WT- wild type) AND examine the effect of mutations that block the function of: 1. The a factor receptor (STE2 yeast) 2. The cell arrest factor (FAR1 yeast) Wild Type STE mutation FAR mutation Alpha factor Alpha factor Alpha factor G Protein coupled receptor G Proin coupled receptor G Protein coupled receptor 1 G Protein G Protein G Protein Kinase complex Kinase complex Kinase complex Kinase Kinase Kinase Cell Arrest Factor Transcription (Budding?) (Schmooing?) Cell Arrest Factor Transcription (Budding?) (Schmooing?) Ceny rest Factor Transcription udding (Schmooing?) Predictions 1.Based on the signaling pathway described, what do you expect normal (wild type) yeast cells to do in the presence and absence of alpha factor (bud/schmoo/both/neither)? 2.What do you expect a type yeast cells that have no arrest factor (FAR2) to do in the presence and absence of alpha factor (bud/schmoo/both/neither)? 3.What do you expect a type yeast cells that have alpha factor receptor (STE1) to do in the presence and absence of alpha factor (bud/schmoo/both/neither)?

Answer 1

Ans 1 As the wild type pathway suggest in presence of Alpha factor yeast will show both budding and schmooing while in the absence of Alpha factor yeast will neither show both budding and schmooing.

Ans 2 As the Far mutant type pathway suggest in presence of Alpha factor yeast will show only schmooing while in the absence of Alpha factor yeast will neither show both budding and schmooing. As cell arrest fator is non functional.

Ans 3 As the STE mutant type pathway suggest in presence of Alpha factor yeast will neither show both budding and schmooing as well as in  the absence of Alpha factor yeast will neither show both budding and schmooing. As Alpha factor is nonfunctional.