Describe the roles of DNA polymerase I, DNA polymerase III, gyrase, helicase, primase, and ligase in the replication of E. coli DNA. What features of the E. coli replisome and of DNA polymerase III’s structure are associated with replication processivity? How do mammalian cells prime discontinuous strand replication and how do they remove RNA primers?
Describe the roles of DNA polymerase I, DNA polymerase III, gyrase, helicase, primase, and ligase in...
(Molecular Biology) Define the following components of replication: polymerase, helicase, DNA ligase, RNA primase, and ssDNA binding proteins.
In DNA replication ywhat are the leading and lagging strand synthesis plus the activities of DNA helicase, DNA gyrase, DNA polymerase, RNA primase and DNA ligase
1a.) Which of the following characteristics is not associated with E. coli primase? A.) it synthesizes the RNA primer in DNA replication B.) it synthesizes a primer with a free 3'−OH end C.) it is essential for DNA replication D.) it is essential for RNA replication 1b.) Which of the following is not required for optimal DNA replication? A.) Gyrase B.) Primase C.) Single strand binding proteins. D.) All of these are necessary. E.) DNA Polymerase II.
Describe DNA replication on the leading and lagging strands of DNA. Your answer must include a description of the roles of the following enzymes: -DNA polymerase I (5’-3’polymerase activity, 3’-5’ exonuclease activity, 5’-3’ exonuclease activity) -DNA polymerase III (5’-3’polymerase activity, 3’-5’ exonuclease activity) -helicase, primase, ligase, gyrase
Below is a diagram of a DNA molecule that is undergoing bidirectional replication. On the diagram label primers, Okasaki fragments, and the site of action of the enzymes: DNA polymerase III, DNA polymerase III, DNA ligase, DNA helicase and DNA gyrase. Show the polarities (5' rightarrow 3') of the daughter strands. Be sure to label where replication is continuous or discontinuous.
D Question 1 (Matching) Match the enzyme/structure with its role in DNA replication. DNA polymerase synthesizes the new strand of RNA primase Choose synthesizes the new strand of DNA synthesizes a short fragment of complementary RNA attach to template DNA strands to prevent hydrogen bonding Uncoils the supercoil of prokaryotic chromosomes connects Okazaki fragments of new lagging strand of DNA unwinds and unzips the double stranded DNA Single stranded binding proteins (SSB's) DNA Ligase [Choose] Helicase [Choose) DNA Gyrase (Choose]...
Define termsDNA, RNA, nucleotides, plasmid, helicase, DNA polymerase, primase, RNA primer of DNA replication, mutation, gene, amino acid, polypeptide chain, protein, codon, promoter region of a gene, RNA polymerase, transcription, mRNA, tRNA, RNA, ribosomes, translation, gene expression, conjugation, conjugative pilus, transformation, transductionExplain concept or process• Describe how nucleotides are linked together to form a single strand of nucleic acid• Explain the concept of a complementary pairing • Describe how DNA replication occurs in bacteria • Explain why a primer is necessary for...
Describe the process of DNA replication. (Your answer should include the following: replication fork, semiconservative replication, replication fork, DNA gyrase, helicase, primase, DNA polymerase, DNA ligase, leading strand, lagging strand, continuous replication, non-continuous replication, and Okazaki fragment)
Which of the following statements about DNA replication is FALSE? Primase synthesizes the primers. DNA polymerase is required to add new nucleotides to the growing ends of the DNA strands. DNA ligase joins the small DNA fragments of the lagging strand. Only one strand of the parent DNA serves as a template for a newly synthesized complementary strand.
5 Describe the process of DNA replication; include the following terms: antiparallel structure, DNA Okazaki fragments, DNA ligase, primer, primase, helicase, topoisomerase, single-strand binding proteins. Describe the function of Helicase, Primase, topoisomerase, DNA Polymerase III, DNA Polymerase I, DNA ligase