In a lacI- strain of E. coli (the repressor protein is not expressed in this mutant)...
In a lacI- strain of E. coli (the repressor protein is not expressed in this mutant) the lac operon will be expressed...
Homework Answers
In a lacI- strain of E. coli, the lac operon will be expressed both in the presence or absence of lactose.
The expression will be constitutive because the transcription of lac structural genes will not be inhibited when lacI mutation prevents the lac repressor protein from binding to the operator.
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In a lacI- strain of E. coli (the repressor protein is not
expressed in this mutant)...
help please as soon as possible A mutant E. coli strain is found that does not...
help please as soon as possible
A mutant E. coli strain is found that does not synthesize any enzymes in the presence or absence of lactose (or allolactose). W mutation(s) can lead to this outcome? The lac operon is shown here as a guide. fones where proteins bind DNA genes and regulatory sequences Operator La repressor B-galactosidase B-galactos de transactylase proteins promoter Operator Lacy Lac A laci o Lacz
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences I lacl promoter operator lac Z l ac Y La repressor B-galactosidase B-galactoside transacetylase proteins Operator Laci promoter Lac Y Lac A Lacz
the answer I gave was wrong A mutant E. coli strain is found that synthesizes B-galactosidase...
the answer I gave was wrong
A mutant E. coli strain is found that synthesizes B-galactosidase whether or not lactose (or allolactose) is present. What mutations can independently lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences lac 1 operator promoter -35 lac z lac Y lac A JOSSA expression Lac repressor B-galactosidase B-galactoside- transacetylase proteins permease Lac Z Lac A promoter operator laci Lac Y
Consider the following lac operon genotype: laci+P+OCZY+A+ In an E. coli strain with this genotype, what...
Consider the following lac operon genotype: laci+P+OCZY+A+ In an E. coli strain with this genotype, what is the status of beta- galactosidase activity in the PRESENCE of lactose? O A. - OB. + c. Can't tell
consider a strain of E. coli that has a mutation in the gene encoding the IIA...
consider a strain of E. coli that has a mutation in the gene encoding the IIA protein of the phosphotransferase system. The transport and phosphorylation of glucose is not affected by this mutation and the phosphate group is transferred to glucose as in the wildtype strain. Also, the interaction between the mutant IIA protein and Lacy is not affected by the mutation. However, the mutation does affect the IIA protein's interactions with adenylate cyclase and the phosphorylated form of the...
Allolactose acts as a signal molecule that binds to the LacI protein (repressor) and prevents it...
Allolactose acts as a signal molecule that binds to the LacI protein (repressor) and prevents it from binding to the Operator on the lac operon. But if the repressor is on the operator, no B-glactosidase can be made which means no allolactose can be made in the first place. So how is the repressor removed so allolactose can be made to stop the repressor from binding?
B2. Consider E. coli cells, each having one of the following mutations: a) a mutant lac...
B2. Consider E. coli cells, each having one of the following mutations: a) a mutant lac operator (Oc locus) that cannot bind repressor. b) a mutant lac repressor (I- gene product) that cannot bind to the lac operator. c) a mutant lac repressor (the Is gene product) that cannot bind to lactose. d) a mutant lac promoter that cannot bind CAP + cAMP. What effect would each mutation have on the function of lac operon (assuming no glucose is present)...
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene....
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene. The production of B-galactosidase (+ present or - absent) is measured when lactose is present and absent from the medium. Assume the mutations involve only 1, 0, or Z. A merodiploid is constructed for each of the three strains. The plasmid carries a wild type lac operon and lacl gene. The production of functional B-galactosidase (+ present or - absent) is measured when lactose...
As a student project, you have isolated six new mutant strains of E. coli with altered...
As a student project, you have isolated six new mutant strains
of E. coli with altered behavior of the lactose operon. The strains
are listed in the table below, together with their phenotypes (with
regard to significant ?-galactosidase synthesis) in three specific
situations.
Columns 1 and 2 present the phenotypes of each mutant haploid
strain. In column 1, the mutant is in an otherwise wild-type
genome. In column 2, the genome also carries a nonsense-suppressor
mutation (that is not present...
20862a a A mutant E. coli strain is found that does not synthesize any enzymes in...
20862a a A mutant E. coli strain is found that does not synthesize any enzymes in the presence or absence of lactose for allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. ones where proteins bind DNA genes and regulatory sequences och Operator o Lacy o Lacz o Laca promoter MacBook Air * जीप
help please as soon as possible
A mutant E. coli strain is found that does not synthesize any enzymes in the presence or absence of lactose (or allolactose). W mutation(s) can lead to this outcome? The lac operon is shown here as a guide. fones where proteins bind DNA genes and regulatory sequences Operator La repressor B-galactosidase B-galactos de transactylase proteins promoter Operator Lacy Lac A laci o Lacz
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences I lacl promoter operator lac Z l ac Y La repressor B-galactosidase B-galactoside transacetylase proteins Operator Laci promoter Lac Y Lac A Lacz
the answer I gave was wrong
A mutant E. coli strain is found that synthesizes B-galactosidase whether or not lactose (or allolactose) is present. What mutations can independently lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences lac 1 operator promoter -35 lac z lac Y lac A JOSSA expression Lac repressor B-galactosidase B-galactoside- transacetylase proteins permease Lac Z Lac A promoter operator laci Lac Y
Consider the following lac operon genotype: laci+P+OCZY+A+ In an E. coli strain with this genotype, what is the status of beta- galactosidase activity in the PRESENCE of lactose? O A. - OB. + c. Can't tell
consider a strain of E. coli that has a mutation in the gene encoding the IIA protein of the phosphotransferase system. The transport and phosphorylation of glucose is not affected by this mutation and the phosphate group is transferred to glucose as in the wildtype strain. Also, the interaction between the mutant IIA protein and Lacy is not affected by the mutation. However, the mutation does affect the IIA protein's interactions with adenylate cyclase and the phosphorylated form of the...
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene. The production of B-galactosidase (+ present or - absent) is measured when lactose is present and absent from the medium. Assume the mutations involve only 1, 0, or Z. A merodiploid is constructed for each of the three strains. The plasmid carries a wild type lac operon and lacl gene. The production of functional B-galactosidase (+ present or - absent) is measured when lactose...
As a student project, you have isolated six new mutant strains
of E. coli with altered behavior of the lactose operon. The strains
are listed in the table below, together with their phenotypes (with
regard to significant ?-galactosidase synthesis) in three specific
situations.
Columns 1 and 2 present the phenotypes of each mutant haploid
strain. In column 1, the mutant is in an otherwise wild-type
genome. In column 2, the genome also carries a nonsense-suppressor
mutation (that is not present...
20862a a A mutant E. coli strain is found that does not synthesize any enzymes in the presence or absence of lactose for allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. ones where proteins bind DNA genes and regulatory sequences och Operator o Lacy o Lacz o Laca promoter MacBook Air * जीप
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