Question

Part . Essay Question Answer the Anver the Bollawing quetions y optimal ollowing velocity d asyod under the optimal conditions and the Departsonen og Biological S 1. The enayme A was assay were obtained: ollowing velocity data aM mol L/min A. R 2. The 1 0.05 2 0.12 4 0.4 6 1.2 10 2.5 20 4 40 4.2 Assume Vnax for the enzyme to be 4.5 nmol/L/min a. whether it follows Michaelis-Menten kinetics or not; if not determine the type of cooperativity (from saturation plot and Koshland coefficient) b. calculate Sool So.i proportion (Koshland coefficient) c. determine from the appropriate plots, Km, or Kd' and Hill coefficient

Answer 1