Question

how can I(immunhistochemistry) IHC be used to judge the virulence of 2 neuro-invasive virus strains in mouse infections?

can u also reference a good website or paper that explains it also?

Answer 1

Viruses generally insert their genome to the host cell genome and when the host genome multiplies, the viral genome also multiply. The viral envelope, made by proteins also produced by using the host amino acids. Now we should produce or select some antibodies which have potentiality to bind with those viral envelope protein and link them with fluorescent markers. Fluorescent markers easily emits some light when they bind with the antigen. Now the antibodies are applied by vesicles to the nerve cell, suspected as the virus affected. If the nerve cell fluoresce some light or colour then the cell is supposed as virus infection. The intensity of the light/colour from the nerve cell indicates to the amount of viral lode.

You can get the mechanism at the book of immunology - Kuby.