Question

ATP h movement can involve either DNA or RNA elements betwees recombining molecules of seguence homology d almost ahways resuts in lethal mutacions 23. Poly-A tal of mRNA a added as soon as the 5 end emerges from the RNA polbmerase h is part of the gametic oode coetained in the DNA s required fbr mRNA to be esported out of the mucless 24 All ofthe following may be found at the eucaryotic rplication fork esoept a DNA polymerase h. DNA primasome c sliding ring protein d DNA helicase е.clamp loader protein 25. "Bulky lesion a region of distorted DNA double helix signaling need for mucleotide excision repair h another term for the chromosothe "puff observed in polytene chromosomes c the shape of the RNA polymerase tail 6lowing phosphorylation e skin rash that foms in response to excess studying for biology exama 26. All of the following conceming the eucaryotic spliceosome is trus, EXCEPT a fonctions in the cytoscl h oore consists of small RNA molecules, each complexed with several proteins c catalytic sites are composed of RNA d. differest type of splicesotne for each class of mRNA molecule e diffierent parts of the splicesome ester and leave the complex as the splicing reactions proceed 7 Sythesis of a DNA molecule requires d all of these (a b, and c) e a and b only b. DNA helicase с.RNA Which of the folowing concerning primary cuture cells is false wwally lack selomerase may be derived from cancerous tumors ly replicate only 10-40 gmerations d. may suffier from "culture shock" e may be used to create a new cel line
Please answer all questions. Some are a bit blurry but if you zoom in you can read them. I need all of these to study. #21 all of the following are required for polymerase 2 to operate,except

#22 site-specific recombinstion:

Answer 1

Please find the answers below:

Answer 21: Choice a (chromatin modifying proteins are required for epigenetic modifications in the DNA, not the activity of any polymerase)

Answer 22: Choice c (site-specific recombination refers to the type of genetic recombination between specific locations of the DNA, hence requires lateral sequence homogenity between the target sequences)

Answer 23: Choice e (the poly-A tail is required for protection of mRNA as well as its transport)

Answer 24: Choice b (the enzyme primase and the related machinery are required for initiation of repliation, hence not a mandatory part of replication fork)

Answer 25: Choice b (the bulkly lesions are generally observed during DNA replication in polytene chromosomes as large unwound DNA)

Answer 26: Choice e (once all the proteins and nucleic acid components of the spliceosome machinery are in place, they bring about alteration in the target sequence, without lateral movements in or out)

Answer 27: Choice d (all these enzymes are required for DNA replication, ranging from opening of the helix, stabilization of the strain and initiation of replication)

Answer 28: Choice c (unlike established cell lines, the primary cultures are short-lived in nature and replicate to only 8-10 generations at maximum, hence experiments are conducted very quickly once the primary cells have been harvested)