Osmotic Pressure question: Looking for the molar weight of protein with the following numbers:
Started with a sample of 1.928 grams of protein
30 degrees Celcius 66.3 mATM
40 mL of water
what is the molar weight of protein in g/mol???
*Gives a hint that the weight should be 10^4 - 10^6 g/mol
P = MRT
for
m = 1.928 g of protein
T= 30°C = 303 K
P = 66.3*10^-3 atm
V = 40*10^-3 L
so
substitute
M = P/(RT) = (66.3*10^-3) / (0.082*303) = 0.0026684 mol per liter
mol = MV = 0.0026684 * 40*10^-3 = 0.000106736 mol
MW = mass/mol = (1.928) / (0.000106736 = 18063.2588 which is in the reange of 10^4
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