A permissive bacterial strain is simultaneously infected with two strains of T4- like phage. One phage strain has the genotype a -b + , while the other is a +b - . If the map distance between markers a and b is about 10 map units, what proportion of the progeny phages are expected to have the genotype a +b +?
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A permissive bacterial strain is simultaneously infected with
two strains of T4- like phage. One phage...
QUESTION 6 8 points Save Answer A bacterial strain with the genotype pro galt lysstrain was...
QUESTION 6 8 points Save Answer A bacterial strain with the genotype pro galt lysstrain was used as the donor to transform another strain with the genotype pro gal lys'. The genotypes of the transformed cells were as follows: Number of cells Genotype pro gals pro galthy progally pro galthy pro gals jo gally frogatys What is the order of the genes? Please explain your reasoning. Which two genes are closest to each other? How do you know? QUESTION 5...
E. coli strain B is doubly infected with two rII mutants of phage T4. 0.1 ml...
E. coli strain B is doubly infected with two rII mutants of phage T4. 0.1 ml of a 10^6 dilution of the progeny is plated on E. coli B and 0.1 ml of a 10^5 dilution of the progeny is plated on E. coli K. 400 plaques appeared on strain B, 10 on strain K. Calculate the map distance between these two mutations. Please show detailed work to demonstrate where each number comes from and state your reasoning. A. 0.05...
recombination in phages you have isolated two mutations that affect plaque morphology in phages (y and...
recombination in phages
you have isolated two mutations that affect plaque
morphology in phages (y and z). you conduct a genetic cross between
the two phage strains by coinfecting bacteria with a wild-type
strain and a strain that carries both mutations. phage chromosomes
enter the host and replicate. you collect some of the progeny
phages and plate them onto a lawn of bacterial cells. you observe
the following numbers of plaques:
1. which are examples of recombinant progeny?
Select all...
QUESTION 3 Vulcans have three genes, pointed ears (P), absent adrenal glands (A), and a right-sided...
QUESTION 3 Vulcans have three genes, pointed ears (P), absent adrenal glands (A), and a right-sided heart (R) which are on the same chromosome, as shown below. The gene P is 10 map units from A. The gene A is 25 map units from R. All three of these alleles are dominant to normal Earth alleles: rounded ears (p), adrenal glands (a) and a left sided heart (r). P. (10 m.u.) A (25 m.u.) R Mr. Spock, the first officer...
here are the original strains: please answer C, D, E, and F with full solutions, thank...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
5. Using Pl as a generalised transducing phage grown on a cys' pur trp" bacterial donor,...
5. Using Pl as a generalised transducing phage grown on a cys' pur trp" bacterial donor, a recipient strain of genotype cys pur* trp was infected and incubated. In experiment Icys transductants were selected and in experiment II trp* transductants wer,selected. (Note: cys, trp and pur genes are involved in cysteine, tryptophan and purine biosynthesis respectively) (a) What media were used for these selection experiments? Next, the transductants were examined for the presence of the unselected donor markers (genes), with...
You have three bacterial strains: one is resistant to kanamycin (strain A), another is resistant to...
You have three bacterial strains: one is resistant to kanamycin (strain A), another is resistant to gentamycin (strain B), and a third is not resistant to either antibiotic, but produces a purple pigment (strain C). (A) Suppose the antibiotic resistance of one strain is due to a gene on the chromosome, whereas the resistance of the other is due to a gene on a conjugative plasmid. Describe how you could determine which antibiotic resistance is plasmid encoded. (B) Describe how...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
3. (7) Suppose that you have selected E. coli that can grow only when the medium...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
A cross was made between two strains of plants that are agriculturally important. One strain was...
A cross was made between two strains of plants that are agriculturally important. One strain was disease-resistant but herbicide-sensitive; the other strain was disease-sensitive but herbicide- resistant. A plant breeder crossed the two plants and then allowed the F1 generation to self-fertilize. The following data were obtained: F1 generation: All offspring were disease sensitive and herbicide resistant. F2 generation: 170 disease sensitive, herbicide resistant 60 disease sensitive, herbicide sensitive 44 disease resistant, herbicide resistant 10 disease resistant, herbicide sensitive Formulate...
QUESTION 6 8 points Save Answer A bacterial strain with the genotype pro galt lysstrain was used as the donor to transform another strain with the genotype pro gal lys'. The genotypes of the transformed cells were as follows: Number of cells Genotype pro gals pro galthy progally pro galthy pro gals jo gally frogatys What is the order of the genes? Please explain your reasoning. Which two genes are closest to each other? How do you know? QUESTION 5...
recombination in phages
you have isolated two mutations that affect plaque
morphology in phages (y and z). you conduct a genetic cross between
the two phage strains by coinfecting bacteria with a wild-type
strain and a strain that carries both mutations. phage chromosomes
enter the host and replicate. you collect some of the progeny
phages and plate them onto a lawn of bacterial cells. you observe
the following numbers of plaques:
1. which are examples of recombinant progeny?
Select all...
QUESTION 3 Vulcans have three genes, pointed ears (P), absent adrenal glands (A), and a right-sided heart (R) which are on the same chromosome, as shown below. The gene P is 10 map units from A. The gene A is 25 map units from R. All three of these alleles are dominant to normal Earth alleles: rounded ears (p), adrenal glands (a) and a left sided heart (r). P. (10 m.u.) A (25 m.u.) R Mr. Spock, the first officer...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
5. Using Pl as a generalised transducing phage grown on a cys' pur trp" bacterial donor, a recipient strain of genotype cys pur* trp was infected and incubated. In experiment Icys transductants were selected and in experiment II trp* transductants wer,selected. (Note: cys, trp and pur genes are involved in cysteine, tryptophan and purine biosynthesis respectively) (a) What media were used for these selection experiments? Next, the transductants were examined for the presence of the unselected donor markers (genes), with...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
A cross was made between two strains of plants that are agriculturally important. One strain was disease-resistant but herbicide-sensitive; the other strain was disease-sensitive but herbicide- resistant. A plant breeder crossed the two plants and then allowed the F1 generation to self-fertilize. The following data were obtained: F1 generation: All offspring were disease sensitive and herbicide resistant. F2 generation: 170 disease sensitive, herbicide resistant 60 disease sensitive, herbicide sensitive 44 disease resistant, herbicide resistant 10 disease resistant, herbicide sensitive Formulate...
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