E. coli strain B is doubly infected with two rII mutants of phage T4. 0.1 ml...
E. coli strain B is doubly infected with two rII mutants of phage T4. 0.1 ml of a 10^6 dilution of the progeny is plated on E. coli B and 0.1 ml of a 10^5 dilution of the progeny is plated on E. coli K. 400 plaques appeared on strain B, 10 on strain K. Calculate the map distance between these two mutations.
Please show detailed work to demonstrate where each number comes from and state your reasoning.
A. 0.05 mu
B. 0.1 mu
C. 0.2 mu
D. 0.5 mu
E. 1.0 mu
Homework Answers
Add Answer to:
E. coli strain B is doubly infected with two rII mutants of
phage T4. 0.1 ml...
A permissive bacterial strain is simultaneously infected with two strains of T4- like phage. One phage...
A permissive bacterial strain is simultaneously infected with
two strains of T4- like phage. One phage strain has the genotype a
-b + , while the other is a +b - . If the map distance between
markers a and b is about 10 map units, what proportion of the
progeny phages are expected to have the genotype a +b +?
A permissive bacterial strain is simultaneously infected with two strains of T4- like phage. One phage strain has the...
An Hfr strain is used to map four genes in an interrupted mating experiment. The cross...
An Hfr strain is used to map four genes in an interrupted mating experiment. The cross is Hfr/a+b+c+ rifs ×F−a−b−c− rifr . (No map order is implied in the listing of the alleles; rifr is resistance to the antibiotic rifampicin.) The a+gene is required for the biosynthesis of nutrient A, the b+ gene for nutrient B, c+ for nutrient C. The cross is initiated at time = 0, and at various times, the mating mixture is plated on medium. Each plate contains minimal medium (MM) plus rifampicin....
7) When Crick et al. plated large numbers of T4, with suppression mutations only, on K2...
7) When Crick et al. plated large numbers of T4, with suppression mutations only, on K2 what did they find? a) no plaques b) xITA mutant plaques d) IIB mutant plaques c) plaques containing suppressor of suppressor mutations 8) What are the suppressors of the FCO mutation AND how do they work? a) deletions, by preventing the protein from being made b) other phage, work by complementing c) frameshifts, shift the frame back to the correct frame d ) inversions,...
3. (4) Suppose that you use a phage P1 grown on a strain of E. coli...
3. (4) Suppose that you use a phage P1 grown on a strain of E. coli which is cys+ ton trp- to infect a recipient strain of E. coli which is cys- tons trp+. These bacteria are then plated on complete media with phage T1 (tons sensitive to phage T1). You obtain 50 ton? transductants which you go on to test on minimal media for the other markers. cyst trp+ cyst trp- cys- trpt cys- trp- 10 24 13 a....
You infect E. coli with two strains of T4 bacteriophage with genotypes +B+ and A+C. You...
You infect E. coli with two strains of T4 bacteriophage with genotypes +B+ and A+C. You collect and analyze 1000 plaques, obtaining the following results: ABC = 31 +++ = 36 AB+ = 4 A+C = 410 A++ = 50 +BC = 46 +B+ = 421 ++C = 2 Draw a map with distances for the three genes. Which gene is in the middle? Calculate the interference.
QUESTION 6 8 points Save Answer A bacterial strain with the genotype pro galt lysstrain was...
QUESTION 6 8 points Save Answer A bacterial strain with the genotype pro galt lysstrain was used as the donor to transform another strain with the genotype pro gal lys'. The genotypes of the transformed cells were as follows: Number of cells Genotype pro gals pro galthy progally pro galthy pro gals jo gally frogatys What is the order of the genes? Please explain your reasoning. Which two genes are closest to each other? How do you know? QUESTION 5...
ce%20Problem-520-Sel%205X%281%29 H Tools Add-ons Help Accessibility All changes saved in Drive nal text - Times New......
ce%20Problem-520-Sel%205X%281%29 H Tools Add-ons Help Accessibility All changes saved in Drive nal text - Times New... 12 - B I U A 2- 15 5. Assume that you counted 67 plaques on a bacterial plate where 0.1ml of a 10-5 dilution of phage was added to bacterial culture. What is the initial concentration of the undiluted phage? Show your calculations and give your answer in pfu/ml (pfu - plaque-forming units) 6. E. coli strain B can be infected by phage...
1. You are studying phage transduction and trying to deliver a kanamycin resistance gene from one...
1. You are studying phage transduction and trying to deliver a kanamycin resistance gene from one strain of bacteria to another using phage. The kanR gene is FAR away from the lambda prophage in your bacterium. Choose which type of transduction should you use for your experiment and explain why. a. lambda phage, specialized transduction b. lambda phage, conjugation C. T4 phage, generalized transduction d. A different phage with mitomycin C induction 2. A pig is infected with two subtypes...
A permissive bacterial strain is simultaneously infected with
two strains of T4- like phage. One phage strain has the genotype a
-b + , while the other is a +b - . If the map distance between
markers a and b is about 10 map units, what proportion of the
progeny phages are expected to have the genotype a +b +?
A permissive bacterial strain is simultaneously infected with two strains of T4- like phage. One phage strain has the...
7) When Crick et al. plated large numbers of T4, with suppression mutations only, on K2 what did they find? a) no plaques b) xITA mutant plaques d) IIB mutant plaques c) plaques containing suppressor of suppressor mutations 8) What are the suppressors of the FCO mutation AND how do they work? a) deletions, by preventing the protein from being made b) other phage, work by complementing c) frameshifts, shift the frame back to the correct frame d ) inversions,...
3. (4) Suppose that you use a phage P1 grown on a strain of E. coli which is cys+ ton trp- to infect a recipient strain of E. coli which is cys- tons trp+. These bacteria are then plated on complete media with phage T1 (tons sensitive to phage T1). You obtain 50 ton? transductants which you go on to test on minimal media for the other markers. cyst trp+ cyst trp- cys- trpt cys- trp- 10 24 13 a....
QUESTION 6 8 points Save Answer A bacterial strain with the genotype pro galt lysstrain was used as the donor to transform another strain with the genotype pro gal lys'. The genotypes of the transformed cells were as follows: Number of cells Genotype pro gals pro galthy progally pro galthy pro gals jo gally frogatys What is the order of the genes? Please explain your reasoning. Which two genes are closest to each other? How do you know? QUESTION 5...
ce%20Problem-520-Sel%205X%281%29 H Tools Add-ons Help Accessibility All changes saved in Drive nal text - Times New... 12 - B I U A 2- 15 5. Assume that you counted 67 plaques on a bacterial plate where 0.1ml of a 10-5 dilution of phage was added to bacterial culture. What is the initial concentration of the undiluted phage? Show your calculations and give your answer in pfu/ml (pfu - plaque-forming units) 6. E. coli strain B can be infected by phage...
1. You are studying phage transduction and trying to deliver a kanamycin resistance gene from one strain of bacteria to another using phage. The kanR gene is FAR away from the lambda prophage in your bacterium. Choose which type of transduction should you use for your experiment and explain why. a. lambda phage, specialized transduction b. lambda phage, conjugation C. T4 phage, generalized transduction d. A different phage with mitomycin C induction 2. A pig is infected with two subtypes...
Most questions answered within 3 hours.
-
26) Briefly describe, using words or simple diagrams, the
chemiosmotic theory for coupling oxidation to phosphorylation...
asked 1 hour ago -
Suppose that XX is a random variable with mean 16 and standard
deviation 5 . Also...
asked 1 hour ago -
Calculate the number density of argon gas at a temperature of
24C and a pressure of...
asked 5 hours ago -
Alternative
Classification
How to Estimate
Probabilities from Data? ( For continuous Attributes)
And How to generate...
asked 5 hours ago -
An explosion breaks a 20.0-kg object into three parts. The
object is initially moving at a...
asked 5 hours ago -
Calculate the approximate number of residues of Rubisco, which
is involved in carbon fixation in plants,...
asked 6 hours ago -
Other decisions about scientific claims can have a much broader
impact.ENERGYarrow-10x10.png, environment, health, security - all...
asked 7 hours ago -
I need to write a research paper and work cited about this
topic: The United States...
asked 8 hours ago -
Hello! I was wondering if I could have some help?
If the vapor pressure of carvone...
asked 8 hours ago -
An economist wants to estimate the mean per capita income (in
thousands of dollars) for a...
asked 8 hours ago -
What would be the input/output characteristic of a circuit
obtained by putting two of your 2's-complementers...
asked 8 hours ago -
In Drosophila, the transition from the syncytial blastoderm
stage to the cellular blastoderm stage is a...
asked 9 hours ago