You infect E. coli with two strains of T4 bacteriophage with genotypes +B+ and A+C. You...
You infect E. coli with two strains of T4 bacteriophage with genotypes +B+ and A+C. You collect and analyze 1000 plaques, obtaining the following results:
ABC = 31
+++ = 36
AB+ = 4
A+C = 410
A++ = 50
+BC = 46
+B+ = 421
++C = 2
Draw a map with distances for the three genes. Which gene is in the middle? Calculate the interference.
Homework Answers
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You infect E. coli with two strains of T4 bacteriophage with
genotypes +B+ and A+C. You...
E. coli strain B is doubly infected with two rII mutants of phage T4. 0.1 ml...
E. coli strain B is doubly infected with two rII mutants of phage T4. 0.1 ml of a 10^6 dilution of the progeny is plated on E. coli B and 0.1 ml of a 10^5 dilution of the progeny is plated on E. coli K. 400 plaques appeared on strain B, 10 on strain K. Calculate the map distance between these two mutations. Please show detailed work to demonstrate where each number comes from and state your reasoning. A. 0.05...
Three Hfr strains of E. coli, each with genotype StrS, lacZ+, A, B, C, D are...
Three Hfr strains of E. coli, each with genotype StrS, lacZ+, A, B, C, D are mated to an F- strain with the genotype StrR, lacZ-, a, b, c, d. Samples of conjugating strains are collected and disrupted every minute for one hour using a blender. Cells are plated under conditions that allow the lacZ+, A, B, C, and D alleles to be identified. The time at which each dominant allele is transferred is shown in the table. lacZ A...
here are the original strains: please answer C, D, E, and F with full solutions, thank...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
3. (7) Suppose that you have selected E. coli that can grow only when the medium...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
1. You have a plant with three different genes, a, b, and c. These genes are...
1. You have a plant with three different genes, a, b, and c. These genes are on the same chromosome and c is in the middle. The distance between a and c is 6 map units and the distance between c and b is 14 map units. You have a plant, heterozygous for all three genes as shown below: a C F + D You cross this plant in a test cross and collect 1000 progeny plants. (48 points) How...
Assignment #1 1. You have a plant with three different genes, a, b, and c. These...
Assignment #1 1. You have a plant with three different genes, a, b, and c. These genes are on the same chromosome and c is in the middle. The distance between a and c is 6 map units and the distance between c and bis 14 map units. You have a plant, heterozygous for all three genes as shown below: ac FFD You cross this plant in a test cross and collect 1000 progeny plants. (48 points) How many progenies...
You are following the inheritance of three genes: A, B and C. Aand B are...
You are following the inheritance of three genes: A, B and C. A and B are on the same chromosome; C is on a different chromosome from A and B (for example; A and B are on chromosome 1 and C is on chromosome 4).Which option correctly represents the genotype of an individual who is heterozygous for all three genes?What is the recombination frequency for unlinked genes (i.e., genes that sort independently)? Frequency is given as a number from 0...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
You have three genes on the same chromosome - A, B and C. Each gene has...
You have three genes on the same chromosome - A, B and C. Each gene has two alleles in a dominant/recessive relationship. For these genes the homozygous recessive has the mutant phenotype for that trait, the dominant phenotype = wild type for that trait. allele A is dominant to a; phenotype a = mutant for trait a; phenotype A = wild type for trait A allele B is dominant to b; phenotype b = mutant for trait b; phenotype B...
You have three genes on the same chromosome - A, B and C. Each gene has...
You have three genes on the same chromosome - A, B and C. Each gene has two alleles in a dominant/recessive relationship. For these genes the homozygous recessive has the mutant phenotype for that trait, the dominant phenotype = wild type for that trait. allele A is dominant to a; phenotype a = mutant for trait a; phenotype A = wild type for trait A allele B is dominant to b; phenotype b = mutant for trait b; phenotype B...
here are the original strains:
please answer C, D, E, and F with full solutions, thank you!
:)
C) When you conduct an interrupted mating experiment with the Hfr and F-strains, Ton colonies with the following phenotypes first appear at the times shown in the table below: Phenotype Minutes cys+ cys- 25 gal+ 17 38.5 9.5 pur+ 14.5 trp+ his+ lac+ 25 Draw a bacterial chromosome and indicate the relative position of each of the markers and the cysX gene...
3. (7) Suppose that you have selected E. coli that can grow only
when the medium is supplemented with cysteine. You isolate one
mutant that you believe is defective in a new gene require for
cysteine biosynthesis - cysX .
You have two strains of bacteria:
Hfr cysX - gal+ his+ lac+ pur+ trp+ ton s
F - cysX+ gal - his - lac - pur - trp - ton R
A) What supplements would you need to add to...
1. You have a plant with three different genes, a, b, and c. These genes are on the same chromosome and c is in the middle. The distance between a and c is 6 map units and the distance between c and b is 14 map units. You have a plant, heterozygous for all three genes as shown below: a C F + D You cross this plant in a test cross and collect 1000 progeny plants. (48 points) How...
Assignment #1 1. You have a plant with three different genes, a, b, and c. These genes are on the same chromosome and c is in the middle. The distance between a and c is 6 map units and the distance between c and bis 14 map units. You have a plant, heterozygous for all three genes as shown below: ac FFD You cross this plant in a test cross and collect 1000 progeny plants. (48 points) How many progenies...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
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